In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions Under Flow Conditions

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Abstract

The exit of leukocytes from the bloodstream into inflamed sites involves a sequence of interactions with vascular endothelial cells, in which leukocytes, moving rapidly in flowing blood, first tether and roll on the endothelial surface before arresting and then transmigrating across the endothelial barrier. Examining the mechanisms of these interactions in human systems has involved the use of in vitro flow chamber assays, using a variety of cells and immobilized molecules as adhesive substrata. Here we describe how to perform these assays using human umbilical vein endothelial cells and human leukocytes.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric F. Morand
PublisherHumana Press
Chapter2
Pages19-25
Number of pages7
Volume2080
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adhesion
  • Flow chamber
  • Leukocyte interactions
  • Rolling
  • Shear conditions
  • Transmigration

Cite this

Hickey, M. J. (2020). In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions Under Flow Conditions. In J. Harris, & E. F. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (Vol. 2080, pp. 19-25). (Methods in molecular biology ; Vol. 2080). Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_2