In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates

Jennifer Susan Collins; Joji Tanaka; Paul J Wilson; Kristian Kempe; Thomas Paul Davis; Michelle Paula McIntosh; Michael Raymond Whittaker; David Mark Haddleton

doi:10.1021/bc5006202

In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates

Jennifer Susan Collins, Joji Tanaka, Paul J Wilson, Kristian Kempe, Thomas Paul Davis, Michelle Paula McIntosh, Michael Raymond Whittaker, David Mark Haddleton

Drug Delivery Disposition & Dynamics

Research output: Contribution to journal › Article › Research › peer-review

51 Citations (Scopus)

Abstract

The in situ one-pot synthesis of peptide-polymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.

Original language	English
Pages (from-to)	633-638
Number of pages	6
Journal	Bioconjugate Chemistry
Volume	26
Issue number	4
DOIs	https://doi.org/10.1021/bc5006202
Publication status	Published - 2015

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10.1021/bc5006202

3497050-oaFinal published version, 1.93 MB

Macromolecular design for bio-imaging and targeted delivery
Davis, T. (Primary Chief Investigator (PCI))
Australian Research Council (ARC), Monash University
1/07/14 → 31/12/19
Project: Research
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology
Davis, T. (Primary Chief Investigator (PCI)), Boyd, B. (Chief Investigator (CI)), Bunnett, N. (Chief Investigator (CI)), Porter, C. (Chief Investigator (CI)), Caruso, F. (Chief Investigator (CI)), Kent, S. (Chief Investigator (CI)), Thordarson, P. (Chief Investigator (CI)), Kearnes, M. (Chief Investigator (CI)), Gooding, J. (Chief Investigator (CI)), Kavallaris, M. (Chief Investigator (CI)), Thurecht, K. J. (Chief Investigator (CI)), Whittaker, A. K. (Chief Investigator (CI)), Parton, R. (Chief Investigator (CI)), Corrie, S. R. (Chief Investigator (CI)), Johnston, A. (Chief Investigator (CI)), McGhee, J. (Chief Investigator (CI)), Greguric, I. D. (Partner Investigator (PI)), Stevens, M. M. (Partner Investigator (PI)), Lewis, J. S. (Partner Investigator (PI)), Lee, D. S. (Partner Investigator (PI)), Alexander, C. (Partner Investigator (PI)), Dawson, K. (Partner Investigator (PI)), Hawker, C. (Partner Investigator (PI)), Haddleton, D. (Partner Investigator (PI)), Thierry, B. (Chief Investigator (CI)), Prestidge, C. A. (Chief Investigator (CI)), Meyer, A. (Project Manager), Jones-Jayasinghe, N. (Project Manager), Voelcker, N. (Chief Investigator (CI)), Nann, T. (Chief Investigator (CI)) & McLean, K. (Partner Investigator (PI))
Australian Research Council (ARC), Monash University, University of Melbourne, University of New South Wales (UNSW), University of Queensland , University of South Australia, Monash University – Internal Faculty Contribution, University of Wisconsin Madison, Memorial Sloan Kettering Cancer Center, University of California System, University College Dublin, Imperial College London, University of Warwick, Sungkyunkwan University, Australian Nuclear Science and Technology Organisation (ANSTO) , University of Nottingham
30/06/14 → 29/06/21
Project: Research

Cite this

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title = "In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates",

abstract = "The in situ one-pot synthesis of peptide-polymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.",

author = "Collins, {Jennifer Susan} and Joji Tanaka and Wilson, {Paul J} and Kristian Kempe and Davis, {Thomas Paul} and McIntosh, {Michelle Paula} and Whittaker, {Michael Raymond} and Haddleton, {David Mark}",

year = "2015",

doi = "10.1021/bc5006202",

language = "English",

volume = "26",

pages = "633--638",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

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T1 - In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates

AU - Collins, Jennifer Susan

AU - Tanaka, Joji

AU - Wilson, Paul J

AU - Kempe, Kristian

AU - Davis, Thomas Paul

AU - McIntosh, Michelle Paula

AU - Whittaker, Michael Raymond

AU - Haddleton, David Mark

PY - 2015

Y1 - 2015

N2 - The in situ one-pot synthesis of peptide-polymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.

AB - The in situ one-pot synthesis of peptide-polymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.

U2 - 10.1021/bc5006202

DO - 10.1021/bc5006202

M3 - Article

SN - 1043-1802

VL - 26

SP - 633

EP - 638

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 4

ER -

In situ conjugation of dithiophenol maleimide polymers and oxytocin for stable and reversible polymer-peptide conjugates

Abstract

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Projects

Macromolecular design for bio-imaging and targeted delivery

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Cite this