In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

Alessia C.G. Weiss, Kilian Krüger, Quinn A. Besford, Mathias Schlenk, Kristian Kempe, Stephan Förster, Frank Caruso

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with preadsorbed proteins, and phase 3, reversibly bound "soft" protein corona proteins. Additionally, we investigate particle-protein interactions on low-fouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles, only a "soft" protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments.

Original languageEnglish
Pages (from-to)2459-2469
Number of pages11
JournalACS Applied Materials and Interfaces
Volume11
Issue number2
DOIs
Publication statusPublished - 2019

Keywords

  • adsorption
  • kinetics
  • low-fouling
  • nanoengineering
  • particles

Cite this

Weiss, Alessia C.G. ; Krüger, Kilian ; Besford, Quinn A. ; Schlenk, Mathias ; Kempe, Kristian ; Förster, Stephan ; Caruso, Frank. / In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics. In: ACS Applied Materials and Interfaces. 2019 ; Vol. 11, No. 2. pp. 2459-2469.
@article{e02d3bbc70034ecfb0d1cc1d4a072e3d,
title = "In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics",
abstract = "In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising {"}hard{"} and {"}soft{"} protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with preadsorbed proteins, and phase 3, reversibly bound {"}soft{"} protein corona proteins. Additionally, we investigate particle-protein interactions on low-fouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles, only a {"}soft{"} protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments.",
keywords = "adsorption, kinetics, low-fouling, nanoengineering, particles",
author = "Weiss, {Alessia C.G.} and Kilian Kr{\"u}ger and Besford, {Quinn A.} and Mathias Schlenk and Kristian Kempe and Stephan F{\"o}rster and Frank Caruso",
year = "2019",
doi = "10.1021/acsami.8b14307",
language = "English",
volume = "11",
pages = "2459--2469",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "ACS Publications",
number = "2",

}

In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics. / Weiss, Alessia C.G.; Krüger, Kilian; Besford, Quinn A.; Schlenk, Mathias; Kempe, Kristian; Förster, Stephan; Caruso, Frank.

In: ACS Applied Materials and Interfaces, Vol. 11, No. 2, 2019, p. 2459-2469.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

AU - Weiss, Alessia C.G.

AU - Krüger, Kilian

AU - Besford, Quinn A.

AU - Schlenk, Mathias

AU - Kempe, Kristian

AU - Förster, Stephan

AU - Caruso, Frank

PY - 2019

Y1 - 2019

N2 - In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with preadsorbed proteins, and phase 3, reversibly bound "soft" protein corona proteins. Additionally, we investigate particle-protein interactions on low-fouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles, only a "soft" protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments.

AB - In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding the protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with preadsorbed proteins, and phase 3, reversibly bound "soft" protein corona proteins. Additionally, we investigate particle-protein interactions on low-fouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles, only a "soft" protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments.

KW - adsorption

KW - kinetics

KW - low-fouling

KW - nanoengineering

KW - particles

UR - http://www.scopus.com/inward/record.url?scp=85059697743&partnerID=8YFLogxK

U2 - 10.1021/acsami.8b14307

DO - 10.1021/acsami.8b14307

M3 - Article

VL - 11

SP - 2459

EP - 2469

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 2

ER -