In silico identification of novel toxin homologs and associated mobile genetic elements in clostridium perfringens

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Clostridium perfringens causes a wide range of diseases in a variety of hosts, due to the production of a diverse set of toxins and extracellular enzymes. The C. perfringens toxins play an important role in pathogenesis, such that the presence and absence of the toxins is used as a typing scheme for the species. In recent years, several new toxins have been discovered that have been shown to be essential or highly correlated to diseases; these include binary enterotoxin (BecAB), NetB and NetF. In the current study, genome sequence analysis of C. perfringens isolates from diverse sources revealed several putative novel toxin homologs, some of which appeared to be associated with potential mobile genetic elements, including transposons and plasmids. Four novel toxin homologs encoding proteins related to the pore-forming Leukocidin/Hemolysin family were found in type A and G isolates. Two novel toxin homologs encoding proteins related to the epsilon aerolysin-like toxin family were identified in Type A and F isolates from humans, contaminated food and turkeys. A novel set of proteins related to clostridial binary toxins was also identified. While phenotypic characterisation is required before any of these homologs can be established as functional toxins, the in silico identification of these novel homologs on mobile genetic elements suggests the potential toxin reservoir of C. perfringens may be much larger than previously thought.

Original languageEnglish
Article number16
Number of pages14
JournalPathogens
Volume8
Issue number1
DOIs
Publication statusPublished - 29 Jan 2019

Keywords

  • Binary toxin
  • Clostridium perfringens
  • Epsilon
  • Hemolysin
  • Leukotoxin
  • PCP13
  • PCW3
  • Plasmid
  • Toxin

Cite this

@article{c639bdeb292f40c68bd297d5881aef34,
title = "In silico identification of novel toxin homologs and associated mobile genetic elements in clostridium perfringens",
abstract = "Clostridium perfringens causes a wide range of diseases in a variety of hosts, due to the production of a diverse set of toxins and extracellular enzymes. The C. perfringens toxins play an important role in pathogenesis, such that the presence and absence of the toxins is used as a typing scheme for the species. In recent years, several new toxins have been discovered that have been shown to be essential or highly correlated to diseases; these include binary enterotoxin (BecAB), NetB and NetF. In the current study, genome sequence analysis of C. perfringens isolates from diverse sources revealed several putative novel toxin homologs, some of which appeared to be associated with potential mobile genetic elements, including transposons and plasmids. Four novel toxin homologs encoding proteins related to the pore-forming Leukocidin/Hemolysin family were found in type A and G isolates. Two novel toxin homologs encoding proteins related to the epsilon aerolysin-like toxin family were identified in Type A and F isolates from humans, contaminated food and turkeys. A novel set of proteins related to clostridial binary toxins was also identified. While phenotypic characterisation is required before any of these homologs can be established as functional toxins, the in silico identification of these novel homologs on mobile genetic elements suggests the potential toxin reservoir of C. perfringens may be much larger than previously thought.",
keywords = "Binary toxin, Clostridium perfringens, Epsilon, Hemolysin, Leukotoxin, PCP13, PCW3, Plasmid, Toxin",
author = "Lacey, {Jake A.} and Johanesen, {Priscilla A.} and Dena Lyras and Moore, {Robert J.}",
year = "2019",
month = "1",
day = "29",
doi = "10.3390/pathogens8010016",
language = "English",
volume = "8",
journal = "Pathogens",
issn = "2076-0817",
publisher = "MDPI AG",
number = "1",

}

In silico identification of novel toxin homologs and associated mobile genetic elements in clostridium perfringens. / Lacey, Jake A.; Johanesen, Priscilla A.; Lyras, Dena; Moore, Robert J.

In: Pathogens, Vol. 8, No. 1, 16, 29.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - In silico identification of novel toxin homologs and associated mobile genetic elements in clostridium perfringens

AU - Lacey, Jake A.

AU - Johanesen, Priscilla A.

AU - Lyras, Dena

AU - Moore, Robert J.

PY - 2019/1/29

Y1 - 2019/1/29

N2 - Clostridium perfringens causes a wide range of diseases in a variety of hosts, due to the production of a diverse set of toxins and extracellular enzymes. The C. perfringens toxins play an important role in pathogenesis, such that the presence and absence of the toxins is used as a typing scheme for the species. In recent years, several new toxins have been discovered that have been shown to be essential or highly correlated to diseases; these include binary enterotoxin (BecAB), NetB and NetF. In the current study, genome sequence analysis of C. perfringens isolates from diverse sources revealed several putative novel toxin homologs, some of which appeared to be associated with potential mobile genetic elements, including transposons and plasmids. Four novel toxin homologs encoding proteins related to the pore-forming Leukocidin/Hemolysin family were found in type A and G isolates. Two novel toxin homologs encoding proteins related to the epsilon aerolysin-like toxin family were identified in Type A and F isolates from humans, contaminated food and turkeys. A novel set of proteins related to clostridial binary toxins was also identified. While phenotypic characterisation is required before any of these homologs can be established as functional toxins, the in silico identification of these novel homologs on mobile genetic elements suggests the potential toxin reservoir of C. perfringens may be much larger than previously thought.

AB - Clostridium perfringens causes a wide range of diseases in a variety of hosts, due to the production of a diverse set of toxins and extracellular enzymes. The C. perfringens toxins play an important role in pathogenesis, such that the presence and absence of the toxins is used as a typing scheme for the species. In recent years, several new toxins have been discovered that have been shown to be essential or highly correlated to diseases; these include binary enterotoxin (BecAB), NetB and NetF. In the current study, genome sequence analysis of C. perfringens isolates from diverse sources revealed several putative novel toxin homologs, some of which appeared to be associated with potential mobile genetic elements, including transposons and plasmids. Four novel toxin homologs encoding proteins related to the pore-forming Leukocidin/Hemolysin family were found in type A and G isolates. Two novel toxin homologs encoding proteins related to the epsilon aerolysin-like toxin family were identified in Type A and F isolates from humans, contaminated food and turkeys. A novel set of proteins related to clostridial binary toxins was also identified. While phenotypic characterisation is required before any of these homologs can be established as functional toxins, the in silico identification of these novel homologs on mobile genetic elements suggests the potential toxin reservoir of C. perfringens may be much larger than previously thought.

KW - Binary toxin

KW - Clostridium perfringens

KW - Epsilon

KW - Hemolysin

KW - Leukotoxin

KW - PCP13

KW - PCW3

KW - Plasmid

KW - Toxin

UR - http://www.scopus.com/inward/record.url?scp=85062367573&partnerID=8YFLogxK

U2 - 10.3390/pathogens8010016

DO - 10.3390/pathogens8010016

M3 - Article

VL - 8

JO - Pathogens

JF - Pathogens

SN - 2076-0817

IS - 1

M1 - 16

ER -