In Ovo Electroporation of Avian Somities

Martin Scaal, Jerome Gros, Cynthia Lesbros, Christophe Marcelle

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72 Citations (Scopus)

Abstract

In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.

Original languageEnglish
Pages (from-to)643-650
Number of pages8
JournalDevelopmental Dynamics
Volume229
Issue number3
DOIs
Publication statusPublished - Mar 2004
Externally publishedYes

Keywords

  • Chich embryo
  • Electroporation
  • GFP
  • Somites

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