In ovo electroporation of avian somites

Martin Scaal, Jerome Gros, Cynthia Lesbros, Christophe Marcelle

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.
Original languageEnglish
Pages (from-to)643 - 650
Number of pages8
JournalDevelopmental Dynamics
Volume229
Issue number3
Publication statusPublished - 2004

Cite this

Scaal, M., Gros, J., Lesbros, C., & Marcelle, C. (2004). In ovo electroporation of avian somites. Developmental Dynamics, 229(3), 643 - 650.
Scaal, Martin ; Gros, Jerome ; Lesbros, Cynthia ; Marcelle, Christophe. / In ovo electroporation of avian somites. In: Developmental Dynamics. 2004 ; Vol. 229, No. 3. pp. 643 - 650.
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Scaal, M, Gros, J, Lesbros, C & Marcelle, C 2004, 'In ovo electroporation of avian somites' Developmental Dynamics, vol. 229, no. 3, pp. 643 - 650.

In ovo electroporation of avian somites. / Scaal, Martin; Gros, Jerome; Lesbros, Cynthia; Marcelle, Christophe.

In: Developmental Dynamics, Vol. 229, No. 3, 2004, p. 643 - 650.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - In ovo electroporation of avian somites

AU - Scaal, Martin

AU - Gros, Jerome

AU - Lesbros, Cynthia

AU - Marcelle, Christophe

PY - 2004

Y1 - 2004

N2 - In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.

AB - In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14991719

M3 - Article

VL - 229

SP - 643

EP - 650

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 3

ER -

Scaal M, Gros J, Lesbros C, Marcelle C. In ovo electroporation of avian somites. Developmental Dynamics. 2004;229(3):643 - 650.