Improved performance of preordered fungal protease-stearic acid biocomposites: enhanced catalytic activity, reusability, and temporal stability

Sumant Phadtare, Chandravanu Dash, Anand Gole, V. P. Vinod, Mala Rao, Murali Sastry

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In an earlier report on fungal protease (F-prot) - fatty acid biocomposite film formation [Gole et al. Anal. Chem. 2000, 72, 4301], it was observed that the biocatalytic activity of the immobilized enzyme was comparable to that of the free enzyme in solution. However, a somewhat negative aspect of the protocol was the steady loss in activity during reuse and storage of the biocomposite film. In this paper, we address the latter issues and demonstrate successful attempts toward the realization of efficient biocomposite films with enhanced biological activity, temporal stability, and excellent reusability. The improved performance of the F-prot - stearic acid biocomposite is accomplished by preordering the fatty acid film by incorporation of Pb2+ ions into the lipid matrix prior to enzyme immobilization. The lead cation induces lamellar ordering in the lipid film and thus facilitates diffusion of the F-prot molecules into the lipid matrix and accessibility of the substrate molecules (hemoglobin, Hb) to the entrapped F-prot enzyme molecules. The preordering consequently leads to effective control of the "mass transport" problem and might be responsible for the enhanced biological activity (∼36%) of the enzyme molecules in the biocomposite in comparison with the free enzyme in solution, as well the excellent reusability of the composite film. In addition to biocatalytic activity measurements, the formation and characterization of the F-prot - lead stearate biocomposite films was done by quartz crystal microgravimetry and X-ray diffraction.

Original languageEnglish
Pages (from-to)700-705
Number of pages6
JournalBiotechnology Progress
Issue number4
Publication statusPublished - 2002
Externally publishedYes

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