TY - CHAP
T1 - Improved embryonic stem cell technologies
AU - Drape, J. S.
AU - Nagy, A.
PY - 2007
Y1 - 2007
N2 - Murine embryonic stem (ES) cells have become an indispensable tool for investigating genetic function both in vitro and, importantly, in vivo. Recent advances, including tetraploid aggregation, new site-specific recombinases and RNAi, have enabled more sophisticated manipulation of the ES cell genome. For instance, it is now possible to control gene expression in both a temporally and spatially restricted manner. Such new technologies are answering complex questions surrounding the function and interaction of an increasing number of genes. This chapter will review both the history and recent technological progress that has been made in mouse ES cell derivation, genetic manipulation and the generation of ES cell-derived chimaeric animals.
AB - Murine embryonic stem (ES) cells have become an indispensable tool for investigating genetic function both in vitro and, importantly, in vivo. Recent advances, including tetraploid aggregation, new site-specific recombinases and RNAi, have enabled more sophisticated manipulation of the ES cell genome. For instance, it is now possible to control gene expression in both a temporally and spatially restricted manner. Such new technologies are answering complex questions surrounding the function and interaction of an increasing number of genes. This chapter will review both the history and recent technological progress that has been made in mouse ES cell derivation, genetic manipulation and the generation of ES cell-derived chimaeric animals.
KW - Chimaera
KW - ES cell
KW - Homologous recombination
KW - Tetraploid complementation
KW - Transgenesis
UR - http://www.scopus.com/inward/record.url?scp=33846939023&partnerID=8YFLogxK
U2 - 10.1007/978-3-540-35109-2-5
DO - 10.1007/978-3-540-35109-2-5
M3 - Chapter (Book)
C2 - 17203653
AN - SCOPUS:33846939023
SN - 9783540351085
VL - 178
T3 - Handbook of Experimental Pharmacology
SP - 107
EP - 128
BT - Conditional Mutagenesis
ER -