Improved detection of mycobacteria species in formalin-fixed tissue sections

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Aims: To develop an antibody broadly reactive against mycobacterial species, which will improve detection of mycobacteria in tissue sections by immunohistochemistry (IHC). Methods: A sheep antisera was developed by immunization with multiple mycobacteria, and was tested by IHC against a range of mycobacteria in tissues from many species, as well as negative tissue controls and other bacteria. Results: The sheep antiserum, MAS-01, reacted with all 18 mycobacterial species tested, but did not react with uninfected inflammatory tissues. Although MAS-01 cross-reacted with two microbial genera which are related to mycobacteria (Corynebacteria and Proprionibacteria), it did not with Nocardia or Actinomyces. The antibody was more sensitive than the Ziehl-Neelsen stain for detection of tissue mycobacteria, and shortened the time required to identify these infections. Conclusion: The MAS-01 antiserum will facilitate rapid identification of tissue mycobacterial infection by histopathologists. 

Original languageEnglish
Pages (from-to)993-1005
Number of pages13
JournalHistopathology
Volume59
Issue number5
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Immunohistochemistry
  • Infection
  • Mycobacteria
  • Tuberculosis

Cite this

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title = "Improved detection of mycobacteria species in formalin-fixed tissue sections",
abstract = "Aims: To develop an antibody broadly reactive against mycobacterial species, which will improve detection of mycobacteria in tissue sections by immunohistochemistry (IHC). Methods: A sheep antisera was developed by immunization with multiple mycobacteria, and was tested by IHC against a range of mycobacteria in tissues from many species, as well as negative tissue controls and other bacteria. Results: The sheep antiserum, MAS-01, reacted with all 18 mycobacterial species tested, but did not react with uninfected inflammatory tissues. Although MAS-01 cross-reacted with two microbial genera which are related to mycobacteria (Corynebacteria and Proprionibacteria), it did not with Nocardia or Actinomyces. The antibody was more sensitive than the Ziehl-Neelsen stain for detection of tissue mycobacteria, and shortened the time required to identify these infections. Conclusion: The MAS-01 antiserum will facilitate rapid identification of tissue mycobacterial infection by histopathologists. ",
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Improved detection of mycobacteria species in formalin-fixed tissue sections. / Pedersen, John S; Clarke, Iain; Mills, John.

In: Histopathology, Vol. 59, No. 5, 11.2011, p. 993-1005.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Improved detection of mycobacteria species in formalin-fixed tissue sections

AU - Pedersen, John S

AU - Clarke, Iain

AU - Mills, John

PY - 2011/11

Y1 - 2011/11

N2 - Aims: To develop an antibody broadly reactive against mycobacterial species, which will improve detection of mycobacteria in tissue sections by immunohistochemistry (IHC). Methods: A sheep antisera was developed by immunization with multiple mycobacteria, and was tested by IHC against a range of mycobacteria in tissues from many species, as well as negative tissue controls and other bacteria. Results: The sheep antiserum, MAS-01, reacted with all 18 mycobacterial species tested, but did not react with uninfected inflammatory tissues. Although MAS-01 cross-reacted with two microbial genera which are related to mycobacteria (Corynebacteria and Proprionibacteria), it did not with Nocardia or Actinomyces. The antibody was more sensitive than the Ziehl-Neelsen stain for detection of tissue mycobacteria, and shortened the time required to identify these infections. Conclusion: The MAS-01 antiserum will facilitate rapid identification of tissue mycobacterial infection by histopathologists. 

AB - Aims: To develop an antibody broadly reactive against mycobacterial species, which will improve detection of mycobacteria in tissue sections by immunohistochemistry (IHC). Methods: A sheep antisera was developed by immunization with multiple mycobacteria, and was tested by IHC against a range of mycobacteria in tissues from many species, as well as negative tissue controls and other bacteria. Results: The sheep antiserum, MAS-01, reacted with all 18 mycobacterial species tested, but did not react with uninfected inflammatory tissues. Although MAS-01 cross-reacted with two microbial genera which are related to mycobacteria (Corynebacteria and Proprionibacteria), it did not with Nocardia or Actinomyces. The antibody was more sensitive than the Ziehl-Neelsen stain for detection of tissue mycobacteria, and shortened the time required to identify these infections. Conclusion: The MAS-01 antiserum will facilitate rapid identification of tissue mycobacterial infection by histopathologists. 

KW - Immunohistochemistry

KW - Infection

KW - Mycobacteria

KW - Tuberculosis

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