TY - JOUR
T1 - Improved accuracy of the transcriptional profiling method of age grading in Aedes aegypti mosquitoes under laboratory and semi-field cage conditions and in the presence of Wolbachia infection
AU - Caragata, Eric
AU - Poinsignon, Anne
AU - Moreira, L
AU - Johnson, P
AU - Leong, Yi San
AU - Ritchie, Scott
AU - O'Neill, Scott Leslie
AU - McGraw, Elizabeth
PY - 2011
Y1 - 2011
N2 - Transcriptional profiling is an effective method of predicting age in the mosquito Aedes aegypti in the laboratory, however, its effectiveness is limited to younger mosquitoes. To address this we used a microarray to identify new gene candidates that show significant expression changes in older mosquitoes. These genes were then used to create a revised model, which upon evaluation in both laboratory and semi-field conditions, proved to have improved accuracy overall and for older mosquitoes. In association with the development of symbiont-based control strategies for Ae. aegypti, we also tested the model s accuracy for Wolbachia-infected mosquitoes and found no decline in performance. Our findings suggest that the new model is a robust and powerful tool for age determination in Australian Ae. aegypti populations.
AB - Transcriptional profiling is an effective method of predicting age in the mosquito Aedes aegypti in the laboratory, however, its effectiveness is limited to younger mosquitoes. To address this we used a microarray to identify new gene candidates that show significant expression changes in older mosquitoes. These genes were then used to create a revised model, which upon evaluation in both laboratory and semi-field conditions, proved to have improved accuracy overall and for older mosquitoes. In association with the development of symbiont-based control strategies for Ae. aegypti, we also tested the model s accuracy for Wolbachia-infected mosquitoes and found no decline in performance. Our findings suggest that the new model is a robust and powerful tool for age determination in Australian Ae. aegypti populations.
UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2583.2010.01059.x/full
U2 - 10.1111/j.1365-2583.2010.01059.x
DO - 10.1111/j.1365-2583.2010.01059.x
M3 - Article
SN - 0962-1075
VL - 20
SP - 215
EP - 224
JO - Insect Molecular Biology
JF - Insect Molecular Biology
IS - 2
ER -