Importin alpha2-interacting proteins with nuclear roles during mammalian spermatogenesis

Jennifer Dung Ly Huynh, Kim Lieu, Andrew Major, Penelope Alexandra Falshaw Whiley, Janet Holt, Katherine Loveland, David Jans

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21 Citations (Scopus)

Abstract

Spermatogenesis, the process of generating haploid sperm capable of fertilizing the female gamete, requires the timely transport into the nucleus of transcription and chromatin remodeling factors, mediated by members of the importin (IMP) super family. Previous IMP expression profiling implies a role for IMPalpha2 in testicular germ cells late in spermatogenesis. To identify interacting proteins of IMPalpha2 that are potential drivers of germ cell development, we performed yeast-two-hybrid screening of an adult mouse testis library. IMPalpha2-interactions were verified by coimmunoprecipation approaches, while immunohistochemical staining of testis sections confirmed their coexpression with IMPalpha2 in specific testicular cell types. Key interactors identified were a novel isoform of a cysteine and histidine rich protein (Chrp), a protein inhibitor of activated STAT (PIAS) family member involved in transcriptional regulation and sumoylation, Androgen receptor interacting protein 3 (Arip3) and Homologous protein 2 (Hop2), known to be involved in homologous chromosome pairing and recombination), all of which are highly expressed in the testis and show mRNA expression profiles similar to that of IMPalpha2 throughout testicular development. This is the first study to identify binding partners of IMPalpha2 in the developmental context of germline development, and we propose that the regulated expression and timely IMPalpha2-mediated nuclear transport of these proteins may coordinate events during spermatogenesis, with IMPalpha2-mediated nuclear localization representing a potentially critical developmental switch in the testis.
Original languageEnglish
Pages (from-to)1191 - 1202
Number of pages12
JournalBiology of Reproduction
Volume85
Issue number6
DOIs
Publication statusPublished - 2011

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