Importance of mediators for lignin degradation by fungal laccase

Lionel F. Longe, Julien Couvreur, Mathilde Leriche Grandchamp, Gil Garnier, Florent Allais, Kei Saito

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Three of the major ligninolytic enzymes, lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (LA), as well as the secretome of a white-rot fungi, Grammothele fuligo, are tested on three industrial lignins (organosolv, alkali, and Kraft), to investigate and study the differences in biodegradation reactions and mechanism of these three lignins. Strategies involving additives in laccase mediated systems were also considered to produce small phenolic compounds. Three new or underreported additives including 2,4,6-tri-tert-butylphenol (TTBP), 4-tert-butyl-2,6-dimethylphenol (TBDMP), and 3-hydroxyanthranilic acid (HAA) are compared to three classic laccase mediators violuric acid (VA), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and 1-hydroxybenzotriazole (1-HBT). Decrease of molecular weight by up to 73% could be obtained on organosolv lignin with LA-VA systems, and by 49%, 43%, and 39% when LA was used with ABTS, TBDMP, and 1-HBT, respectively. In-depth analysis of the degradation products by quantitative 2D HMQC NMR indicated that the oxidation is mediator-dependent and provides new insights on the enzymatic mechanism.

Original languageEnglish
Pages (from-to)10097-10107
Number of pages11
JournalACS Sustainable Chemistry and Engineering
Volume6
Issue number8
DOIs
Publication statusPublished - 6 Aug 2018

Keywords

  • Biodegradation
  • Biomass
  • Degradation
  • Depolymerization
  • Enzymes
  • Laccase
  • Lignin
  • Lignin peroxidase
  • Manganese peroxidase
  • Mediator

Cite this

Longe, Lionel F. ; Couvreur, Julien ; Leriche Grandchamp, Mathilde ; Garnier, Gil ; Allais, Florent ; Saito, Kei. / Importance of mediators for lignin degradation by fungal laccase. In: ACS Sustainable Chemistry and Engineering. 2018 ; Vol. 6, No. 8. pp. 10097-10107.
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abstract = "Three of the major ligninolytic enzymes, lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (LA), as well as the secretome of a white-rot fungi, Grammothele fuligo, are tested on three industrial lignins (organosolv, alkali, and Kraft), to investigate and study the differences in biodegradation reactions and mechanism of these three lignins. Strategies involving additives in laccase mediated systems were also considered to produce small phenolic compounds. Three new or underreported additives including 2,4,6-tri-tert-butylphenol (TTBP), 4-tert-butyl-2,6-dimethylphenol (TBDMP), and 3-hydroxyanthranilic acid (HAA) are compared to three classic laccase mediators violuric acid (VA), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and 1-hydroxybenzotriazole (1-HBT). Decrease of molecular weight by up to 73{\%} could be obtained on organosolv lignin with LA-VA systems, and by 49{\%}, 43{\%}, and 39{\%} when LA was used with ABTS, TBDMP, and 1-HBT, respectively. In-depth analysis of the degradation products by quantitative 2D HMQC NMR indicated that the oxidation is mediator-dependent and provides new insights on the enzymatic mechanism.",
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Importance of mediators for lignin degradation by fungal laccase. / Longe, Lionel F.; Couvreur, Julien; Leriche Grandchamp, Mathilde; Garnier, Gil; Allais, Florent; Saito, Kei.

In: ACS Sustainable Chemistry and Engineering, Vol. 6, No. 8, 06.08.2018, p. 10097-10107.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Importance of mediators for lignin degradation by fungal laccase

AU - Longe, Lionel F.

AU - Couvreur, Julien

AU - Leriche Grandchamp, Mathilde

AU - Garnier, Gil

AU - Allais, Florent

AU - Saito, Kei

PY - 2018/8/6

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KW - Degradation

KW - Depolymerization

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KW - Lignin

KW - Lignin peroxidase

KW - Manganese peroxidase

KW - Mediator

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DO - 10.1021/acssuschemeng.8b01426

M3 - Article

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EP - 10107

JO - ACS Sustainable Chemistry & Engineering

JF - ACS Sustainable Chemistry & Engineering

SN - 2168-0485

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ER -