Impaired resistance to infection does not increase the virulence of Salmonella htrA live vaccines for mice

Karen Strahan, Steve N. Chatfield, John Tite, Gordon Dougan, C. E. Hormaeche

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sublethal whole body irradiation (350 R) or administered rabbit anti-TNFα antiserum, and (CBA/N♀XBALB/c♂) F1 male mice which express the xid sex-linked B cell defect of CBA/N mice and are more susceptible to salmonellae than female littermates. Salmonella typhimurium htrA mutants derived from virulent strains, C5046 (C5htrA::TnphoA) and BRD726 (SL1344 ΔhtrA) were not more invasive in immunosuppressed mice than in normal controls in the three mouse models of defective immunity. The results indicate that susceptibility to S. typhimurium htrA vaccines derived from virulent parents is not enhanced by conditions of impaired resistance to infection.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalMicrobial Pathogenesis
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

Keywords

  • immunity
  • infection
  • mice
  • Salmonella
  • vaccine

Cite this