Impaired Blood Neutrophil Function in the Frequent Exacerbator of Chronic Obstructive Pulmonary Disease: A Proof-of-Concept Study

Arwel Wyn Jones, Richard Robinson, Peer Mohamed, Glen Davison, Hassan Jaysen Izzat, Keir Edward Lewis

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Purpose: The underlying biological mechanisms of the frequent exacerbator phenotype of COPD remain unclear. We compared systemic neutrophil function in COPD patients with or without frequent exacerbations. Methods: Whole blood from COPD frequent exacerbators (defined as ≥2 moderate–severe exacerbations in the previous 2 years) and non-exacerbators (no exacerbations in the preceding 2 years) was assayed for neutrophil function. Neutrophil function in healthy ex-smoking volunteers was also measured as a control (reference) group. Results: A total of 52 subjects were included in this study: 26 frequent exacerbators, 18 non-exacerbators and 8 healthy controls. COPD frequent exacerbators had blunted blood neutrophil fMLP-stimulated oxidative burst compared to both non-exacerbators (p < 0.01) and healthy controls (p < 0.001). There were no differences between COPD frequent exacerbators and non-exacerbators in blood neutrophil PMA-stimulated oxidative burst, but both COPD groups had reduced responses compared to healthy controls (p < 0.001). Bacterial-stimulated neutrophil degranulation was greater in frequent exacerbators than non-exacerbators (p < 0.05). Conclusion: This study is the first to report aberrant receptor-mediated blood neutrophil function in the frequent exacerbator of COPD.

Original languageEnglish
Pages (from-to)881-887
Number of pages7
JournalLung
Volume194
Issue number6
DOIs
Publication statusPublished - Dec 2016
Externally publishedYes

Keywords

  • Chronic obstructive pulmonary disease
  • Elastase
  • Granulocyte
  • Polymorphonuclear leukocytes
  • Reactive oxygen species

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