Projects per year
Abstract
Reactions of ortho-, para- and substituted meta-xylenyl bridged bis(tacn) ligands with two or three molar equivalents of Zn(NO3)2·6H2O at pH 5.0 in the presence of NaClO4 or NH4PF6 resulted in the formation of a range of crystalline zinc complexes. In the presence of perchlorate counter ions the Lpx and Lphx ligands gave rise to dinuclear Zn2+ coordination complexes [Zn2Cl2(Lpx)(H2O)2](ClO4)2(H2O) and [Zn2Cl2(Lphx)(H2O)2](ClO4)2(H2O)2 respectively, which formed infinite 1D polymeric chains. In contrast, in the presence of the hexafluorophosphate counter ion, the Lnix ligand formed a unique tetranuclear Zn2+ complex, [Zn2Cl2(Lnix)(H2O)3](Cl)(PF6)2(H2O)4, the Lcax ligand formed an octanuclear complex [Zn8Cl4(Lcax)4(H2O)10](PF6)6.7(NO3)1.3(H2O)3.3, whilst the Lox ligand formed a mononuclear sandwich structure, [Zn(Lox)](PF6)2(H2O). The protonated metal-free Lpx ligand crystallised as [H4(Lpx)](NO3)2(PF6)(H2O)2 with the stacked ligand cations forming a hydrogen-bonded network. These structural attributes provide an explanation for the significant differences in binding behaviour of recombinant N-terminally tagged proteins with the corresponding Zn2+-azamacrocyclic complexes, compared to the analogous Cu2+- or Ni2+-azamacrocyclic complexes, when immobilised onto support materials and used as immobilised metal affinity chromatographic (IMAC) adsorbents.
Original language | English |
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Pages (from-to) | 187-197 |
Number of pages | 11 |
Journal | Polyhedron |
Volume | 144 |
DOIs | |
Publication status | Published - 1 Apr 2018 |
Keywords
- Bis(tacn) ligands
- Crystal structures
- IMAC separations
- Xylenyl linkers
- Zinc(II) aza-macrocyclic complexes
Projects
- 1 Finished
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Novel Technologies for the Purification of Recombinant Proteins
Hearn, M. (Primary Chief Investigator (PCI)), Spiccia, L. (Chief Investigator (CI)), Christensen, T. (Partner Investigator (PI)), Iversen, L. (Partner Investigator (PI)), Nielsen, P. (Partner Investigator (PI)), Schiodt, C. (Partner Investigator (PI)) & Staby, A. (Partner Investigator (PI))
ARC - Australian Research Council, Novo Nordisk Pharmaceuticals Pty Ltd (Australia), Monash University
30/03/05 → 31/12/08
Project: Research
Equipment
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Australian Synchrotron
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility