Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium

Michelle E Gahan, Diane Webster, Steven Lodewyk Wesselingh, Richard Strugnell

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26 Citations (Scopus)


Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytornegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSFIOIO/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT 153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses. (c) 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1476 - 1483
Number of pages8
Issue number8
Publication statusPublished - 2007

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