Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule

Leanne Dyksterhuis; Jacinta White; M Hickey; Nigel Kirby; S Mudie; Adrian Hawley; A Vashi; Julie Nigro; Jerome Werkmeister; John Ramshaw

doi:10.1016/j.bpj.2011.03.020

Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule

Leanne Dyksterhuis, Jacinta White, M Hickey, Nigel Kirby, S Mudie, Adrian Hawley, A Vashi, Julie Nigro, Jerome Werkmeister, John Ramshaw

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.

Original language	English
Pages (from-to)	2077 - 2083
Number of pages	7
Journal	Biophysical Journal
Volume	100
Issue number	9
DOIs	https://doi.org/10.1016/j.bpj.2011.03.020
Publication status	Published - 2011

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10.1016/j.bpj.2011.03.020

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title = "Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule",

abstract = "We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.",

author = "Leanne Dyksterhuis and Jacinta White and M Hickey and Nigel Kirby and S Mudie and Adrian Hawley and A Vashi and Julie Nigro and Jerome Werkmeister and John Ramshaw",

year = "2011",

doi = "10.1016/j.bpj.2011.03.020",

language = "English",

volume = "100",

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journal = "Biophysical Journal",

issn = "0006-3495",

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T1 - Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule

AU - Dyksterhuis, Leanne

AU - White, Jacinta

AU - Hickey, M

AU - Kirby, Nigel

AU - Mudie, S

AU - Hawley, Adrian

AU - Vashi, A

AU - Nigro, Julie

AU - Werkmeister, Jerome

AU - Ramshaw, John

PY - 2011

Y1 - 2011

N2 - We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.

AB - We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.

UR - http://www.ncbi.nlm.nih.gov/pubmed/21539774

UR - https://www.scopus.com/pages/publications/79959740045

U2 - 10.1016/j.bpj.2011.03.020

DO - 10.1016/j.bpj.2011.03.020

M3 - Article

SN - 0006-3495

VL - 100

SP - 2077

EP - 2083

JO - Biophysical Journal

JF - Biophysical Journal

IS - 9

ER -

Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule

Abstract

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Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule (Biophysical Journal 2011 (100) (2077-2083))

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