Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule

Leanne Dyksterhuis, Jacinta White, M Hickey, Nigel Kirby, S Mudie, Adrian Hawley, A Vashi, Julie Nigro, Jerome Werkmeister, John Ramshaw

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)


We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale.
Original languageEnglish
Pages (from-to)2077 - 2083
Number of pages7
JournalBiophysical Journal
Issue number9
Publication statusPublished - 2011

Cite this