Impact of antigen specificity on CD4+ T cell activation in chronic HIV-1 infection

Miranda Zoe Denham Smith, Sonia Bastidas, Urs Karrer, Annette Oxenius

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Background: HIV infection induces chronic immune activation which is associated with accelerated disease progression; the causes of this activation, however, are incompletely understood. We investigated the activation status of CD4+ T cells specific for chronic herpes viruses and the non-persistent antigen tetanus toxoid (TT) in HIV positive and HIV negative donors to assess whether persistent infections contribute to chronic CD4+ T cell activation.Methods: Untreated HIV+ patients and healthy, aged matched controls were recruited and activation levels assessed and compared between cells specific for persistent and non-persistent antigens. Activation levels on antigen-specific CD4+ T cells were measured by intracellular cytokine staining following in vitro stimulation with various recall antigens (CMV, EBV, HSV, VZV and TT) in conjunction with cell surface phenotyping.Results: Activation levels of herpes virus-specific CD4+ T cell populations, assessed by co-expression of CD38 and HLA-DR, were significantly elevated in HIV+ individuals compared to normal controls and compared to TT-specific responses. In contrast, we found similar levels of activation of TT-specific CD4+ T cells in HIV+ and HIV- donors.Conclusions: These results show a disparate distribution of immune activation within CD4+ T cell populations depending on their specificity and suggest that the elevated level of immune activation that characterizes chronic HIV infection may be influenced by the persistence of other antigens. ? 2013 Smith et al; licensee BioMed Central Ltd.
Original languageEnglish
Pages (from-to)1 - 9
Number of pages9
JournalBMC Infectious Diseases
Volume13
Issue number1 (Art. No.:100)
DOIs
Publication statusPublished - 2013

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