Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain

Iain D. Hay; Kali Gatland; Andrea Campisano; J. Zoe Jordens; Bernd H A Rehm

doi:10.1128/AEM.01078-09

Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain

Iain D. Hay
, Kali Gatland
, Andrea Campisano
, J. Zoe Jordens
, Bernd H A Rehm

Research output: Contribution to journal › Article › Research › peer-review

64 Citations (Scopus)

Abstract

The supermucoid Pseudomonas aeruginosa strain PDO300Δalg8(pBBR1MCS-5: alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

Original language	English
Pages (from-to)	6022-6025
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	18
DOIs	https://doi.org/10.1128/AEM.01078-09
Publication status	Published - Sept 2009
Externally published	Yes

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abstract = "The supermucoid Pseudomonas aeruginosa strain PDO300Δalg8(pBBR1MCS-5: alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.",

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AU - Hay, Iain D.

AU - Gatland, Kali

AU - Campisano, Andrea

AU - Jordens, J. Zoe

AU - Rehm, Bernd H A

PY - 2009/9

Y1 - 2009/9

N2 - The supermucoid Pseudomonas aeruginosa strain PDO300Δalg8(pBBR1MCS-5: alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

AB - The supermucoid Pseudomonas aeruginosa strain PDO300Δalg8(pBBR1MCS-5: alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

UR - https://www.scopus.com/pages/publications/70149102228

U2 - 10.1128/AEM.01078-09

DO - 10.1128/AEM.01078-09

M3 - Article

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AN - SCOPUS:70149102228

SN - 0099-2240

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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ER -

Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain

Abstract

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