Impact of alginate overproduction on attachment and biofilm architecture of a supermucoid Pseudomonas aeruginosa strain

Iain D. Hay, Kali Gatland, Andrea Campisano, J. Zoe Jordens, Bernd H A Rehm

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36 Citations (Scopus)


The supermucoid Pseudomonas aeruginosa strain PDO300Δalg8(pBBR1MCS-5: alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

Original languageEnglish
Pages (from-to)6022-6025
Number of pages4
JournalApplied and Environmental Microbiology
Issue number18
Publication statusPublished - Sep 2009
Externally publishedYes

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