Immunoregulatory activity in adult rat testicular interstitial fluid

Roles of interleukin-1 and transforming growth factor β

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Abstract

Studies on the effect of rat testicular interstitial fluid (IF) on T- cell function have reported both stimulatory and inhibitory actions. Specific cytokines produced within the testis, particularly interleukin-1 (IL-1) and transforming growth factor β (TGFβ), may contribute to these apparently conflicting observations. In proliferation assays employing lectin- or antibody-activated thymocytes or mature T cells in vitro, adult rat testicular IF stimulated T-cell activation and/or proliferation at low assay doses and was inhibitory at higher doses. The stimulatory activity was blocked by recombinant IL-1 receptor antagonist. The inhibitory activity was not affected by a polyspecific TGFβ antiserum. The biological characteristics of the inhibitor were distinct from those of a similar, but considerably less potent, activity in platelet-depleted serum. These data demonstrate that rat testicular IF contains biologically significant concentrations of IL-1 but has a predominantly inhibitory action on T-cell responses. The factor predominantly responsible for this inhibitory activity displays a relatively large apparent molecular weight, is protease sensitive and partially heat labile, but does not appear to be one of the known mammalian TGFβ isoforms.

Original languageEnglish
Pages (from-to)927-934
Number of pages8
JournalBiology of Reproduction
Volume58
Issue number4
DOIs
Publication statusPublished - Apr 1998

Cite this

@article{299cbc9eb53d440087d8636d49315fe8,
title = "Immunoregulatory activity in adult rat testicular interstitial fluid: Roles of interleukin-1 and transforming growth factor β",
abstract = "Studies on the effect of rat testicular interstitial fluid (IF) on T- cell function have reported both stimulatory and inhibitory actions. Specific cytokines produced within the testis, particularly interleukin-1 (IL-1) and transforming growth factor β (TGFβ), may contribute to these apparently conflicting observations. In proliferation assays employing lectin- or antibody-activated thymocytes or mature T cells in vitro, adult rat testicular IF stimulated T-cell activation and/or proliferation at low assay doses and was inhibitory at higher doses. The stimulatory activity was blocked by recombinant IL-1 receptor antagonist. The inhibitory activity was not affected by a polyspecific TGFβ antiserum. The biological characteristics of the inhibitor were distinct from those of a similar, but considerably less potent, activity in platelet-depleted serum. These data demonstrate that rat testicular IF contains biologically significant concentrations of IL-1 but has a predominantly inhibitory action on T-cell responses. The factor predominantly responsible for this inhibitory activity displays a relatively large apparent molecular weight, is protease sensitive and partially heat labile, but does not appear to be one of the known mammalian TGFβ isoforms.",
author = "Hedger, {Mark P.} and Nikolic-Paterson, {David J.} and Paul Hutchinson and Atkins, {Robert C.} and {De Kretser}, {David M.}",
year = "1998",
month = "4",
doi = "10.1095/biolreprod58.4.927",
language = "English",
volume = "58",
pages = "927--934",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

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TY - JOUR

T1 - Immunoregulatory activity in adult rat testicular interstitial fluid

T2 - Roles of interleukin-1 and transforming growth factor β

AU - Hedger, Mark P.

AU - Nikolic-Paterson, David J.

AU - Hutchinson, Paul

AU - Atkins, Robert C.

AU - De Kretser, David M.

PY - 1998/4

Y1 - 1998/4

N2 - Studies on the effect of rat testicular interstitial fluid (IF) on T- cell function have reported both stimulatory and inhibitory actions. Specific cytokines produced within the testis, particularly interleukin-1 (IL-1) and transforming growth factor β (TGFβ), may contribute to these apparently conflicting observations. In proliferation assays employing lectin- or antibody-activated thymocytes or mature T cells in vitro, adult rat testicular IF stimulated T-cell activation and/or proliferation at low assay doses and was inhibitory at higher doses. The stimulatory activity was blocked by recombinant IL-1 receptor antagonist. The inhibitory activity was not affected by a polyspecific TGFβ antiserum. The biological characteristics of the inhibitor were distinct from those of a similar, but considerably less potent, activity in platelet-depleted serum. These data demonstrate that rat testicular IF contains biologically significant concentrations of IL-1 but has a predominantly inhibitory action on T-cell responses. The factor predominantly responsible for this inhibitory activity displays a relatively large apparent molecular weight, is protease sensitive and partially heat labile, but does not appear to be one of the known mammalian TGFβ isoforms.

AB - Studies on the effect of rat testicular interstitial fluid (IF) on T- cell function have reported both stimulatory and inhibitory actions. Specific cytokines produced within the testis, particularly interleukin-1 (IL-1) and transforming growth factor β (TGFβ), may contribute to these apparently conflicting observations. In proliferation assays employing lectin- or antibody-activated thymocytes or mature T cells in vitro, adult rat testicular IF stimulated T-cell activation and/or proliferation at low assay doses and was inhibitory at higher doses. The stimulatory activity was blocked by recombinant IL-1 receptor antagonist. The inhibitory activity was not affected by a polyspecific TGFβ antiserum. The biological characteristics of the inhibitor were distinct from those of a similar, but considerably less potent, activity in platelet-depleted serum. These data demonstrate that rat testicular IF contains biologically significant concentrations of IL-1 but has a predominantly inhibitory action on T-cell responses. The factor predominantly responsible for this inhibitory activity displays a relatively large apparent molecular weight, is protease sensitive and partially heat labile, but does not appear to be one of the known mammalian TGFβ isoforms.

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U2 - 10.1095/biolreprod58.4.927

DO - 10.1095/biolreprod58.4.927

M3 - Article

VL - 58

SP - 927

EP - 934

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -