Abstract
Chronic immune activation persists despite antiretroviral therapy (ART) in HIV+ individuals and underpins an increased risk of age-related co-morbidities. We assessed the Frailty Index in older HIV+ Australian men on ART. Immunometabolic markers on monocytes and T cells were analyzed using flow cytometry, plasma innate immune activation markers by ELISA, and lipidomic profiling by mass spectrometry. The study population consisted of 80 HIV. + men with a median age of 59 (IQR, 56-65), and most had an undetectable viral load (92%). 24% were frail, and 76% were non-frail. Frailty was associated with elevated Glucose transporter-1 (Glut1) expression on the total monocytes (p = 0.04), increased plasma levels of innate immune activation marker sCD163 (OR, 4.8; CI 1.4-15.9, p = 0.01), phosphatidylethanolamine PE(36:3) (OR, 5.1; CI 1.7-15.5, p = 0.004) and triacylglycerol TG(16:1_18:1_18:1) (OR, 3.4; CI 1.3-9.2, p = 0.02), but decreased expression of GM3 ganglioside, GM3(d18:1/18:0) (OR, 0.1; CI 0.0-0.6, p = 0.01) and monohexosylceramide HexCerd(d18:1/22:0) (OR, 0.1; CI 0.0-0.5, p = 0.004). There is a strong inverse correlation between quality of life and the concentration of PE(36:3) (ρ = -0.33, p = 0.004) and PE(36:4) (ρ = -0.37, p = 0.001). These data suggest that frailty is associated with increased innate immune activation and abnormal lipidomic profile. These markers should be investigated in larger, longitudinal studies to determine their potential as biomarkers for frailty.
| Original language | English |
|---|---|
| Pages (from-to) | 112-121 |
| Number of pages | 10 |
| Journal | EBioMedicine |
| Volume | 22 |
| DOIs | |
| Publication status | Published - Aug 2017 |
Keywords
- Aging
- Frailty
- Glut1
- HIV
- Immunometabolism
- Inflammation
- Lipids
- Metabolism
- Monocytes
Cite this
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Immunometabolic and Lipidomic Markers Associated With the Frailty Index and Quality of Life in Aging HIV+ Men on Antiretroviral Therapy. / Yeoh, Hui Ling; Cheng, Allen C.; Cherry, Catherine L.; Weir, Jacquelyn M; Meikle, Peter J.; Hoy, Jennifer F.; Crowe, Suzanne M.; Palmer, Clovis S.
In: EBioMedicine, Vol. 22, 08.2017, p. 112-121.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Immunometabolic and Lipidomic Markers Associated With the Frailty Index and Quality of Life in Aging HIV+ Men on Antiretroviral Therapy
AU - Yeoh, Hui Ling
AU - Cheng, Allen C.
AU - Cherry, Catherine L.
AU - Weir, Jacquelyn M
AU - Meikle, Peter J.
AU - Hoy, Jennifer F.
AU - Crowe, Suzanne M.
AU - Palmer, Clovis S.
PY - 2017/8
Y1 - 2017/8
N2 - Chronic immune activation persists despite antiretroviral therapy (ART) in HIV+ individuals and underpins an increased risk of age-related co-morbidities. We assessed the Frailty Index in older HIV+ Australian men on ART. Immunometabolic markers on monocytes and T cells were analyzed using flow cytometry, plasma innate immune activation markers by ELISA, and lipidomic profiling by mass spectrometry. The study population consisted of 80 HIV. + men with a median age of 59 (IQR, 56-65), and most had an undetectable viral load (92%). 24% were frail, and 76% were non-frail. Frailty was associated with elevated Glucose transporter-1 (Glut1) expression on the total monocytes (p = 0.04), increased plasma levels of innate immune activation marker sCD163 (OR, 4.8; CI 1.4-15.9, p = 0.01), phosphatidylethanolamine PE(36:3) (OR, 5.1; CI 1.7-15.5, p = 0.004) and triacylglycerol TG(16:1_18:1_18:1) (OR, 3.4; CI 1.3-9.2, p = 0.02), but decreased expression of GM3 ganglioside, GM3(d18:1/18:0) (OR, 0.1; CI 0.0-0.6, p = 0.01) and monohexosylceramide HexCerd(d18:1/22:0) (OR, 0.1; CI 0.0-0.5, p = 0.004). There is a strong inverse correlation between quality of life and the concentration of PE(36:3) (ρ = -0.33, p = 0.004) and PE(36:4) (ρ = -0.37, p = 0.001). These data suggest that frailty is associated with increased innate immune activation and abnormal lipidomic profile. These markers should be investigated in larger, longitudinal studies to determine their potential as biomarkers for frailty.
AB - Chronic immune activation persists despite antiretroviral therapy (ART) in HIV+ individuals and underpins an increased risk of age-related co-morbidities. We assessed the Frailty Index in older HIV+ Australian men on ART. Immunometabolic markers on monocytes and T cells were analyzed using flow cytometry, plasma innate immune activation markers by ELISA, and lipidomic profiling by mass spectrometry. The study population consisted of 80 HIV. + men with a median age of 59 (IQR, 56-65), and most had an undetectable viral load (92%). 24% were frail, and 76% were non-frail. Frailty was associated with elevated Glucose transporter-1 (Glut1) expression on the total monocytes (p = 0.04), increased plasma levels of innate immune activation marker sCD163 (OR, 4.8; CI 1.4-15.9, p = 0.01), phosphatidylethanolamine PE(36:3) (OR, 5.1; CI 1.7-15.5, p = 0.004) and triacylglycerol TG(16:1_18:1_18:1) (OR, 3.4; CI 1.3-9.2, p = 0.02), but decreased expression of GM3 ganglioside, GM3(d18:1/18:0) (OR, 0.1; CI 0.0-0.6, p = 0.01) and monohexosylceramide HexCerd(d18:1/22:0) (OR, 0.1; CI 0.0-0.5, p = 0.004). There is a strong inverse correlation between quality of life and the concentration of PE(36:3) (ρ = -0.33, p = 0.004) and PE(36:4) (ρ = -0.37, p = 0.001). These data suggest that frailty is associated with increased innate immune activation and abnormal lipidomic profile. These markers should be investigated in larger, longitudinal studies to determine their potential as biomarkers for frailty.
KW - Aging
KW - Frailty
KW - Glut1
KW - HIV
KW - Immunometabolism
KW - Inflammation
KW - Lipids
KW - Metabolism
KW - Monocytes
UR - http://www.scopus.com/inward/record.url?scp=85025694076&partnerID=8YFLogxK
U2 - 10.1016/j.ebiom.2017.07.015
DO - 10.1016/j.ebiom.2017.07.015
M3 - Article
VL - 22
SP - 112
EP - 121
JO - EBioMedicine
JF - EBioMedicine
SN - 2352-3964
ER -