Immunology by numbers

Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.
Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalCurrent Opinion in Immunology
Volume40
DOIs
Publication statusPublished - Jun 2016

Cite this

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title = "Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!",
abstract = "We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.",
author = "Purcell, {Anthony W} and Croft, {Nathan P} and Tscharke, {David C}",
year = "2016",
month = "6",
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language = "English",
volume = "40",
pages = "88--95",
journal = "Current Opinion in Immunology",
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publisher = "Elsevier",

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Immunology by numbers : Quantitation of antigen presentation completes the quantitative milieu of systems immunology! / Purcell, Anthony W; Croft, Nathan P; Tscharke, David C.

In: Current Opinion in Immunology, Vol. 40, 06.2016, p. 88-95.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

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T2 - Quantitation of antigen presentation completes the quantitative milieu of systems immunology!

AU - Purcell, Anthony W

AU - Croft, Nathan P

AU - Tscharke, David C

PY - 2016/6

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N2 - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

AB - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.

UR - http://www.ncbi.nlm.nih.gov/pubmed/27060633

U2 - 10.1016/j.coi.2016.03.007

DO - 10.1016/j.coi.2016.03.007

M3 - Review Article

VL - 40

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EP - 95

JO - Current Opinion in Immunology

JF - Current Opinion in Immunology

SN - 0952-7915

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