Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 88-95 |
| Number of pages | 8 |
| Journal | Current Opinion in Immunology |
| Volume | 40 |
| DOIs | |
| Publication status | Published - Jun 2016 |
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Immunology by numbers : Quantitation of antigen presentation completes the quantitative milieu of systems immunology! / Purcell, Anthony W; Croft, Nathan P; Tscharke, David C.
In: Current Opinion in Immunology, Vol. 40, 06.2016, p. 88-95.Research output: Contribution to journal › Review Article › Research › peer-review
TY - JOUR
T1 - Immunology by numbers
T2 - Quantitation of antigen presentation completes the quantitative milieu of systems immunology!
AU - Purcell, Anthony W
AU - Croft, Nathan P
AU - Tscharke, David C
PY - 2016/6
Y1 - 2016/6
N2 - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.
AB - We review approaches to quantitate antigen presentation using a variety of biological and biochemical readouts and highlight the emerging role of mass spectrometry (MS) in defining and quantifying MHC-bound peptides presented at the cell surface. The combination of high mass accuracy in the determination of the molecular weight of the intact peptide of interest and its signature pattern of fragmentation during tandem MS provide an unambiguous and definitive identification. This is in contrast to the potential receptor cross-reactivity towards closely related peptides and variable dose responsiveness seen in biological readouts. In addition, we gaze into the not too distant future where big data approaches in MS can be accommodated to quantify whole immunopeptidomes both in vitro and in vivo.
UR - http://www.ncbi.nlm.nih.gov/pubmed/27060633
U2 - 10.1016/j.coi.2016.03.007
DO - 10.1016/j.coi.2016.03.007
M3 - Review Article
VL - 40
SP - 88
EP - 95
JO - Current Opinion in Immunology
JF - Current Opinion in Immunology
SN - 0952-7915
ER -