Immunolocalisation and activity of DDAH I and II in the heart and modification post-myocardial infarction

Gillian A Gray, Mark Patrizio, Lorcan Sherry, Alyson Miller, Mohammed Malaki, Alison F Wallace, James M Leiper, Patrick Vallance

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15 Citations (Scopus)

Abstract

Asymmetric dimethylarginine (ADMA) and NG monomethyl-l-arginine (l-NMMA) are endogenous inhibitors of nitric oxide synthases (NOS) and their local concentration is determined by the activity of dimethylarginine dimethylaminohydrolases (DDAHs). The current study in male Wistar rats was designed to immunolocalise DDAH I and II in relation to NOS and to investigate changes in distribution, activity and ADMA content in the acute period following myocardial infarction (MI) resulting from coronary artery ligation. Seven days after the coronary artery ligation, l-Arg and methylated arginine content, as well as DDAH activity were determined in homogenates of left ventricular (LV) infarct and border. The distribution of immunoreactive DDAH I, DDAH II, eNOS and iNOS were determined in sections of LV. In healthy hearts, DDAH I was absent, however, DDAH II was localized to endothelium and endocardium with a similar distribution to that of eNOS. Following MI, LV DDAH activity was increased (to 210A?19 of control, P
Original languageEnglish
Pages (from-to)413 - 423
Number of pages11
JournalActa Histochemica
Volume112
Issue number5
DOIs
Publication statusPublished - 2010
Externally publishedYes

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