@article{820e5339e8cf41608da4745537dc30bf,
title = "Immunohistochemical evidence of P2X7R, P2X4R and CaMKK2 in pyramidal neurons of frontal cortex does not align with Alzheimer's disease",
abstract = "Alzheimer's disease (AD) is an incurable neurodegenerative condition resulting in progressive cognitive decline. Pathological features include Aβ plaques, neurofibrillary tangles, neuroinflammation and neuronal death. Purinergic receptors 7 and 4 (P2X7R and P2X4R) and calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) are implicated in neuronal death. We used immunohistochemistry to investigate the distribution of these proteins in neurones from frontal cortex of donors (n = 3/group; aged 79–83 years) who died with and without AD. Neurones were identified morphologically and immunoperoxidase staining was achieved using commercial antibodies. Immunoreactive neurones were counted for each protein by 2–3 raters blinded to the diagnoses. We observed no differences in percentages of P2X7R, P2X4R or CaMKK2 positive neurones (p = 0.2–0.99), but sections from individuals with AD had marginally fewer neurones (p = 0.10). Hence P2X7R, P2X4R or CaMKK2 appear to be expressed in neurones from older donors, but expression does not associate with AD.",
keywords = "Alzheimer's disease, CaMKK2, Neurones, P2X4R, P2X7R",
author = "Jessica Gaff and Connie Jackaman and John Papadimitriou and Shelley Waters and Catriona McLean and Patricia Price",
note = "Funding Information: This research is supported by an Australian Government Research Training Program Scholarship and a Graduate Women of Western Australia Mary and Elsie Stevens Scholarship . The authors acknowledge Curtin University and the Curtin Health Innovation Research Institute for provision of laboratory space and technology platforms, the scientific and technical assistance provided by the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy, Characterisation & Analysis, the University of Western Australia, a facility funded by the University, State and Commonwealth Governments. We acknowledge the provision of tissue from the Victorian Brain Bank, supported by The Florey Institute of Neuroscience and Mental Health, The Alfred and the Victorian Forensic Institute of Medicine and funded in part by the Parkinson's Victoria , MND Victoria FightMND and Yulgilbar Foundation and thank Ms. Fairlie Hinton for facilitating this process. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jun,
doi = "10.1016/j.yexmp.2021.104636",
language = "English",
volume = "120",
journal = "Experimental and Molecular Pathology",
issn = "0014-4800",
publisher = "Elsevier",
}