Immunodiagnosis of paramphistomosis in domestic animals by using dot-ELISA

K. Kaur, S. S. Hassan, K. Joshi, P. D. Juyal

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Indirect dot-ELISA was standardized for detection of paramphistomosis in field bovine sera samples. Random samples (117: adult buffaloes 35, buffalo calves 18, cattle 56, cow calves 8) collected from different regions of Punjab, were tested. Of the total, 93 samples (adult buffaloes=26, buffalo calves=14, cattle=49, cow calves=4) were positive for paramphistome antibodies. The observations were made on the basis of intensity of brown coloured dot formed in positive reaction. The per cent positivity was recorded as very high (+++), high (++), low (+) and mild. Of all the samples, the per cent positivity of adult buffaloes (1=+++, 5=++, 8=+, 12=mild) was 74.28%; buffalo calves (1=+++, 7=++, 1=+, 5=mild) was 77.78%; cattle (2=+++, 11=++, 16=+, 20= mild) was 87.5% and cow carves (2=+,2= mild) was 50%. The overall per cent positivity was found to be 79.48%.

Original languageEnglish
Pages (from-to)650-651
Number of pages2
JournalIndian Journal of Animal Sciences
Issue number6
Publication statusPublished - Jun 2005
Externally publishedYes


  • Dot-ELISA
  • Immunodiagnosis
  • Paramphistomosis

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