Immuno-detection of immature and bioactive forms of the inflammatory cytokine IL-18

Brendan J. Jenkins, Virginie Deswaerte

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review


Gastric cancer (GC) is the third most lethal cancer worldwide, and like many other types of cancers, it is associated with precursory chronic inflammatory responses. In the context of many inflammation-associated cancers such as GC, activation of the innate immune response by infectious microbes and/or host-derived molecules is often characterized by production of the cytokines interleukin (IL)-1β and IL-18, which can often have divergent and opposing (i.e., pro or anti) roles in inflammation and oncogenesis. The processing of these mature bioactive cytokines from their inactive precursor polypeptides is dependent upon the enzyme Caspase-1, which is part of multiprotein complexes called “inflammasomes.” Considering the recent mounting evidence for the role of IL-18 in the pathogenesis of GC, here, we describe a Western blotting technique used on genetic mouse models for GC to detect and characterize both pro-Il-18 and mature IL-18 proteins.

Original languageEnglish
Title of host publicationInflammation and Cancer
Subtitle of host publicationMethods and Protocols
EditorsBrendan J. Jenkins
Place of PublicationNew York, NY
PublisherHumana Press
Number of pages7
ISBN (Electronic)9781493975686
ISBN (Print)9781493975679
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Interleukin (IL)-18
  • Mouse cancer models
  • Tissue lysates
  • Western blotting

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