Immunization of mice with DNA encoding fragment C of tetanus toxin

Richard A Anderson, Xiao Ming Gao, Anastasia Papakonstantinopoulou, Neil Fairweather, Mark Roberts, Gordon Dougan

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19 Citations (Scopus)

Abstract

Immunization of mice with Fragment C protein, the non-toxic C-terminal domain of tetanus toxin, will protect mice against lethal challenge with tetanus toxin. A plasmid, pcDNA3/tetC, which encodes a synthetic tetC gene expressed under the control of the human cytomegalovirus major intermediate early promoter/enhancer region, was constructed. Fragment C expression was observed in Chinese hamster ovary cells following transfection with pcDNA3/tetC. The immune response induced by intramuscular immunization with pure pcDNA3/tetC DNA was evaluated in a murine model. Anti-Fragment C serum immunoglobulin and proliferative responses in splenocytes were observed following two immunizations with pcDNA3/tetC. The major IgG subclass that recognized Fragment C was IgG2a and the stimulated splenocytes secreted high levels of interferon-γ. Sufficient anti-Fragment C serum immunoglobulins were induced by DNA-mediated Immunization to protect mice against lethal challenge with tetanus toxin.

Original languageEnglish
Pages (from-to)827-829
Number of pages3
JournalVaccine
Volume15
Issue number8
DOIs
Publication statusPublished - 1 Jun 1997
Externally publishedYes

Keywords

  • Fragment C protein
  • Immunization
  • Tetanus toxin

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