Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum

William E. Collins, Robin F. Anders, Marguerite Pappaioanou, Gary H. Campbell, Graham V. Brown, David J. Kemp, Ross L. Coppel, Jimmie C. Skinner, Patricia M. Andrysiak, Jenny M. Favaloro, Lynn M. Corcoran, J. Roger Broderson, Graham F. Mitchell, Carlos C. Campbell

Research output: Contribution to journalArticleResearchpeer-review

134 Citations (Scopus)

Abstract

Recent studies have identified and characterized a ring-infected erythrocyte surface antigen (RESA) of the human malaria parasite Plasmodium falciparum with a relative molecular mass (Mr) of ~155,000 (refs 1-7). RESA is localized in the micronemes of merozoites and also the membrane of red cells infected with ring-stage parasites. It is thought to be released through the apical pore from the rhoptry at the time of merozoite invasion 5. Because antibodies directed against this antigen strongly inhibit parasite growth in vitro, RESA may be useful in developing a vaccine against this parasite4,5,7 Here we describe an immunization trial using Aotus monkeys and Escherichia coli-derived fused polypeptides corresponding to various regions of the RESA molecule. Some monkeys in all test groups, but not in the control group, were protected against overwhelming infection. Strikingly, protection correlated with antibody responses to either of two different repetitive sequences in RESA.

Original languageEnglish
Pages (from-to)259-262
Number of pages4
JournalNature
Volume323
Issue number6085
DOIs
Publication statusPublished - 1 Dec 1986

Cite this

Collins, W. E., Anders, R. F., Pappaioanou, M., Campbell, G. H., Brown, G. V., Kemp, D. J., ... Campbell, C. C. (1986). Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum. Nature, 323(6085), 259-262. https://doi.org/10.1038/323259a0
Collins, William E. ; Anders, Robin F. ; Pappaioanou, Marguerite ; Campbell, Gary H. ; Brown, Graham V. ; Kemp, David J. ; Coppel, Ross L. ; Skinner, Jimmie C. ; Andrysiak, Patricia M. ; Favaloro, Jenny M. ; Corcoran, Lynn M. ; Broderson, J. Roger ; Mitchell, Graham F. ; Campbell, Carlos C. / Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum. In: Nature. 1986 ; Vol. 323, No. 6085. pp. 259-262.
@article{d9d3a622b01e43b6becb47b62e1fd711,
title = "Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum",
abstract = "Recent studies have identified and characterized a ring-infected erythrocyte surface antigen (RESA) of the human malaria parasite Plasmodium falciparum with a relative molecular mass (Mr) of ~155,000 (refs 1-7). RESA is localized in the micronemes of merozoites and also the membrane of red cells infected with ring-stage parasites. It is thought to be released through the apical pore from the rhoptry at the time of merozoite invasion 5. Because antibodies directed against this antigen strongly inhibit parasite growth in vitro, RESA may be useful in developing a vaccine against this parasite4,5,7 Here we describe an immunization trial using Aotus monkeys and Escherichia coli-derived fused polypeptides corresponding to various regions of the RESA molecule. Some monkeys in all test groups, but not in the control group, were protected against overwhelming infection. Strikingly, protection correlated with antibody responses to either of two different repetitive sequences in RESA.",
author = "Collins, {William E.} and Anders, {Robin F.} and Marguerite Pappaioanou and Campbell, {Gary H.} and Brown, {Graham V.} and Kemp, {David J.} and Coppel, {Ross L.} and Skinner, {Jimmie C.} and Andrysiak, {Patricia M.} and Favaloro, {Jenny M.} and Corcoran, {Lynn M.} and Broderson, {J. Roger} and Mitchell, {Graham F.} and Campbell, {Carlos C.}",
year = "1986",
month = "12",
day = "1",
doi = "10.1038/323259a0",
language = "English",
volume = "323",
pages = "259--262",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6085",

}

Collins, WE, Anders, RF, Pappaioanou, M, Campbell, GH, Brown, GV, Kemp, DJ, Coppel, RL, Skinner, JC, Andrysiak, PM, Favaloro, JM, Corcoran, LM, Broderson, JR, Mitchell, GF & Campbell, CC 1986, 'Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum', Nature, vol. 323, no. 6085, pp. 259-262. https://doi.org/10.1038/323259a0

Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum. / Collins, William E.; Anders, Robin F.; Pappaioanou, Marguerite; Campbell, Gary H.; Brown, Graham V.; Kemp, David J.; Coppel, Ross L.; Skinner, Jimmie C.; Andrysiak, Patricia M.; Favaloro, Jenny M.; Corcoran, Lynn M.; Broderson, J. Roger; Mitchell, Graham F.; Campbell, Carlos C.

In: Nature, Vol. 323, No. 6085, 01.12.1986, p. 259-262.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum

AU - Collins, William E.

AU - Anders, Robin F.

AU - Pappaioanou, Marguerite

AU - Campbell, Gary H.

AU - Brown, Graham V.

AU - Kemp, David J.

AU - Coppel, Ross L.

AU - Skinner, Jimmie C.

AU - Andrysiak, Patricia M.

AU - Favaloro, Jenny M.

AU - Corcoran, Lynn M.

AU - Broderson, J. Roger

AU - Mitchell, Graham F.

AU - Campbell, Carlos C.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - Recent studies have identified and characterized a ring-infected erythrocyte surface antigen (RESA) of the human malaria parasite Plasmodium falciparum with a relative molecular mass (Mr) of ~155,000 (refs 1-7). RESA is localized in the micronemes of merozoites and also the membrane of red cells infected with ring-stage parasites. It is thought to be released through the apical pore from the rhoptry at the time of merozoite invasion 5. Because antibodies directed against this antigen strongly inhibit parasite growth in vitro, RESA may be useful in developing a vaccine against this parasite4,5,7 Here we describe an immunization trial using Aotus monkeys and Escherichia coli-derived fused polypeptides corresponding to various regions of the RESA molecule. Some monkeys in all test groups, but not in the control group, were protected against overwhelming infection. Strikingly, protection correlated with antibody responses to either of two different repetitive sequences in RESA.

AB - Recent studies have identified and characterized a ring-infected erythrocyte surface antigen (RESA) of the human malaria parasite Plasmodium falciparum with a relative molecular mass (Mr) of ~155,000 (refs 1-7). RESA is localized in the micronemes of merozoites and also the membrane of red cells infected with ring-stage parasites. It is thought to be released through the apical pore from the rhoptry at the time of merozoite invasion 5. Because antibodies directed against this antigen strongly inhibit parasite growth in vitro, RESA may be useful in developing a vaccine against this parasite4,5,7 Here we describe an immunization trial using Aotus monkeys and Escherichia coli-derived fused polypeptides corresponding to various regions of the RESA molecule. Some monkeys in all test groups, but not in the control group, were protected against overwhelming infection. Strikingly, protection correlated with antibody responses to either of two different repetitive sequences in RESA.

UR - http://www.scopus.com/inward/record.url?scp=0022490164&partnerID=8YFLogxK

U2 - 10.1038/323259a0

DO - 10.1038/323259a0

M3 - Article

VL - 323

SP - 259

EP - 262

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6085

ER -

Collins WE, Anders RF, Pappaioanou M, Campbell GH, Brown GV, Kemp DJ et al. Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparum. Nature. 1986 Dec 1;323(6085):259-262. https://doi.org/10.1038/323259a0