Immune privilege of the CNS is not the consequence of limited antigen sampling

Melissa G. Harris, Paul Hulseberg, Changying Ling, Jozsef Karman, Benjamin D. Clarkson, Jeffrey S. Harding, Mengxue Zhang, Adam Sandor, Kelsey Christensen, Andras Nagy, Matyas Sandor, Zsuzsanna Fabry

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43 Citations (Scopus)

Abstract

Central nervous system (CNS) immune privilege is complex, and it is still not understood how CNS antigens are sampled by the peripheral immune system under steady state conditions. To compare antigen sampling from immune-privileged or nonprivileged tissues, we created transgenic mice with oligodendrocyte or gut epithelial cell expression of an EGFP-tagged fusion protein containing ovalbumin (OVA) antigenic peptides and tested peripheral anti-OVA peptide-specific sentinel OT-I and OT-II T cell activation. We report that oligodendrocyte or gut antigens are sampled similarly, as determined by comparable levels of OT-I T cell activation. However, activated T cells do not access the CNS under steady state conditions. These data show that afferent immunity is normally intact as there is no barrier at the antigen sampling level, but that efferent immunity is restricted. To understand how this one-sided surveillance contributes to CNS immune privilege will help us define mechanisms of CNS autoimmune disease initiation.

Original languageEnglish
Article number4422
Number of pages10
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 21 Mar 2014
Externally publishedYes

Keywords

  • immunological surveillance
  • multiple sclerosis

Cite this

Harris, M. G., Hulseberg, P., Ling, C., Karman, J., Clarkson, B. D., Harding, J. S., Zhang, M., Sandor, A., Christensen, K., Nagy, A., Sandor, M., & Fabry, Z. (2014). Immune privilege of the CNS is not the consequence of limited antigen sampling. Scientific Reports, 4, [4422]. https://doi.org/10.1038/srep04422