Immortalization of murine bone marrow-derived macrophages

Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

22 Citations (Scopus)

Abstract

Macrophages are specialized phagocytes that display a variety of important functions for the host immune system. They are particularly important for the recognition of exogenous and endogenous danger signals, forming the defensive front line as part of innate immune response. As such, murine macrophages are commonly used for in vitro cell-based assays examining the mechanisms of innate immune activation, which can require the ongoing breeding and housing of a large number of genetically modified mouse strains. Here, we describe a robust protocol for the generation of immortalized bone marrow-derived macrophages (iBMDMs) from primary murine bone marrow cells. We further provide general protocols for harvesting, freezing, and thawing of bone marrow cells, maintaining iBMDMs in culture and generation of monoclonal iBMDM populations by single-cell cloning.

Original languageEnglish
Title of host publicationMacrophages
Subtitle of host publicationMethods and Protocols
EditorsGermain Rousselet
PublisherHumana Press
Pages35-49
Number of pages15
ISBN (Electronic)9781493978373
ISBN (Print)9781493978366
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1784
ISSN (Print)1064-3745

Keywords

  • Bone marrow-derived macrophages
  • Cre-J2
  • iBMDMs
  • Immortalization
  • Innate immunity
  • Murine macrophages

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