Immortalised Cas9-expressing Cell lines for Gene interrogation

Luis F. Malaver-Ortega, Joseph Rosenbluh

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

1 Citation (Scopus)


The ability of modifying the genome of multiple species, precisely and without or minimal off-targeted effects, have opened numerous opportunities for the biotechnology industry. In this chapter, we describe an easy to establish, robust, and practical pipeline that can be used to generate immortalized cell lines, from different tissues, to capture cell linage context and validate the tools required for genome editing and genetic modification. This pipeline serves as a reference for similar approaches for gene interrogation in other species.

Original languageEnglish
Title of host publicationApplications of Genome Modulation and Editing
EditorsPaul John Verma, Huseyin Sumer, Jun Liu
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages7
ISBN (Electronic)9781071623015
ISBN (Print)9781071623008
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cas9 assay
  • Cell immortalization
  • CRISPR/Cas9
  • Genome editing

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