Immobilized liposome layers for drug delivery applications: Inhibition of angiogenesis

Patrick Vermette, Laurence Meagher, Edith Gagnon, Hans J. Griesser, Charles J. Doillon

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

Liposomes were immobilized onto the surface of perfluorinated polymer tape samples and tissue culture polystyrene well-plates using a multilayer immobilization strategy. In the first step, a thin interfacial bonding layer with surface aldehyde groups was deposited from a glow discharge struck in acetaldehyde vapour. Polyethylenimine was then covalently bound onto the aldehyde groups by reductive amination, followed by covalent binding of NHS-PEG-biotin molecules onto the surface amine groups by carbodiimide chemistry. Next, NeutrAvidin™ protein molecules were bound onto the PEG-biotin layer. Finally, liposomes containing PEG-biotinylated lipids were docked onto the remaining binding sites of the surface-immobilized NeutrAvidin™ molecules. AFM was used to image surface-bound liposomes and revealed a density well below close packing. The release characteristics of the surface-bound liposomes were measured by the fluorescence intensity changes of carboxyfluorescein upon release. Liposomes filled with sodium orthovanadate were surface immobilized and used in two in vitro angiogenesis assays. Marked differences compared to various control samples were observed, demonstrating the utility of drug-filled, surface-bound liposomes for evoking localized, controlled biological host responses proximal to an implanted biomedical device.

Original languageEnglish
Pages (from-to)179-195
Number of pages17
JournalJournal of Controlled Release
Volume80
Issue number1-3
DOIs
Publication statusPublished - 23 Apr 2002
Externally publishedYes

Keywords

  • Angiogenesis
  • Immobilized liposomes
  • Localized drug delivery
  • NeutrAvidin™-biotin interactions

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