Imaging lung aeration and lung liquid clearance at birth

Stuart Brian Hooper, Marcus John Kitchen, Megan Jane Wallace, Naoto Yagi, K Uesugi, Michael John Morgan, Christopher John Hall, Karen Kit Wan Siu, Ivan Michael Williams, Melissa Li-Lian Siew, Sarah Catherine Irvine, Konstantin Mikhailovitch Pavlov, Robert Lewis

Research output: Contribution to journalArticleResearchpeer-review

129 Citations (Scopus)

Abstract

Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that trans-pulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.--Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth.
Original languageEnglish
Pages (from-to)3329 - 3337
Number of pages9
JournalFASEB Journal
Volume21
Issue number12
Publication statusPublished - 2007

Cite this

Hooper, Stuart Brian ; Kitchen, Marcus John ; Wallace, Megan Jane ; Yagi, Naoto ; Uesugi, K ; Morgan, Michael John ; Hall, Christopher John ; Siu, Karen Kit Wan ; Williams, Ivan Michael ; Siew, Melissa Li-Lian ; Irvine, Sarah Catherine ; Pavlov, Konstantin Mikhailovitch ; Lewis, Robert. / Imaging lung aeration and lung liquid clearance at birth. In: FASEB Journal. 2007 ; Vol. 21, No. 12. pp. 3329 - 3337.
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title = "Imaging lung aeration and lung liquid clearance at birth",
abstract = "Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that trans-pulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.--Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth.",
author = "Hooper, {Stuart Brian} and Kitchen, {Marcus John} and Wallace, {Megan Jane} and Naoto Yagi and K Uesugi and Morgan, {Michael John} and Hall, {Christopher John} and Siu, {Karen Kit Wan} and Williams, {Ivan Michael} and Siew, {Melissa Li-Lian} and Irvine, {Sarah Catherine} and Pavlov, {Konstantin Mikhailovitch} and Robert Lewis",
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Hooper, SB, Kitchen, MJ, Wallace, MJ, Yagi, N, Uesugi, K, Morgan, MJ, Hall, CJ, Siu, KKW, Williams, IM, Siew, ML-L, Irvine, SC, Pavlov, KM & Lewis, R 2007, 'Imaging lung aeration and lung liquid clearance at birth', FASEB Journal, vol. 21, no. 12, pp. 3329 - 3337.

Imaging lung aeration and lung liquid clearance at birth. / Hooper, Stuart Brian; Kitchen, Marcus John; Wallace, Megan Jane; Yagi, Naoto; Uesugi, K; Morgan, Michael John; Hall, Christopher John; Siu, Karen Kit Wan; Williams, Ivan Michael; Siew, Melissa Li-Lian; Irvine, Sarah Catherine; Pavlov, Konstantin Mikhailovitch; Lewis, Robert.

In: FASEB Journal, Vol. 21, No. 12, 2007, p. 3329 - 3337.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Imaging lung aeration and lung liquid clearance at birth

AU - Hooper, Stuart Brian

AU - Kitchen, Marcus John

AU - Wallace, Megan Jane

AU - Yagi, Naoto

AU - Uesugi, K

AU - Morgan, Michael John

AU - Hall, Christopher John

AU - Siu, Karen Kit Wan

AU - Williams, Ivan Michael

AU - Siew, Melissa Li-Lian

AU - Irvine, Sarah Catherine

AU - Pavlov, Konstantin Mikhailovitch

AU - Lewis, Robert

PY - 2007

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N2 - Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that trans-pulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.--Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth.

AB - Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that trans-pulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.--Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth.

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M3 - Article

VL - 21

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EP - 3337

JO - FASEB Journal

JF - FASEB Journal

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