Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

David Hartnell; Bradley J. Schwehr; Kate Gillespie-Jones; Dasuni Alwis; Ramesh Rajan; Huishu Hou; Nicole J. Sylvain; Michael J. Pushie; Michael E. Kelly; Massimiliano Massi; Mark J. Hackett

doi:10.1039/d0an00099j

Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

David Hartnell, Bradley J. Schwehr, Kate Gillespie-Jones, Dasuni Alwis, Ramesh Rajan, Huishu Hou, Nicole J. Sylvain, Michael J. Pushie, Michael E. Kelly, Massimiliano Massi, Mark J. Hackett

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation.

Original language	English
Pages (from-to)	3809-3813
Number of pages	5
Journal	Analyst
Volume	145
Issue number	11
DOIs	https://doi.org/10.1039/d0an00099j
Publication status	Published - 7 Jun 2020

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10.1039/d0an00099j

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title = "Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes",

abstract = "The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation. ",

author = "David Hartnell and Schwehr, {Bradley J.} and Kate Gillespie-Jones and Dasuni Alwis and Ramesh Rajan and Huishu Hou and Sylvain, {Nicole J.} and Pushie, {Michael J.} and Kelly, {Michael E.} and Massimiliano Massi and Hackett, {Mark J.}",

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T1 - Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

AU - Hartnell, David

AU - Schwehr, Bradley J.

AU - Gillespie-Jones, Kate

AU - Alwis, Dasuni

AU - Rajan, Ramesh

AU - Hou, Huishu

AU - Sylvain, Nicole J.

AU - Pushie, Michael J.

AU - Kelly, Michael E.

AU - Massi, Massimiliano

AU - Hackett, Mark J.

PY - 2020/6/7

Y1 - 2020/6/7

N2 - The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation.

AB - The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation.

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DO - 10.1039/d0an00099j

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VL - 145

SP - 3809

EP - 3813

JO - Analyst

JF - Analyst

IS - 11

ER -

Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

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Sensory cortex processing changes underlying brain and behaviour deficits caused by traumatic brain injury

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Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

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Sensory cortex processing changes underlying brain and behaviour deficits caused by traumatic brain injury

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