Imaging glucoamylase by scanning tunnelling microscopy

A. Patrick Gunning; Victor J. Morris; Gary Williamson; Nigel J. Belshaw; Gerard F H Kramer; Marja W. Kanning

doi:10.1039/AN9941901939

Imaging glucoamylase by scanning tunnelling microscopy

A. Patrick Gunning, Victor J. Morris, Gary Williamson, Nigel J. Belshaw, Gerard F H Kramer, Marja W. Kanning

Research output: Contribution to journal › Article › Other › peer-review

6 Citations (Scopus)

Abstract

Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

Original language	English
Pages (from-to)	1939-1942
Number of pages	4
Journal	Analyst
Volume	119
Issue number	9
DOIs	https://doi.org/10.1039/AN9941901939
Publication status	Published - 1 Dec 1994
Externally published	Yes

Access to Document

10.1039/AN9941901939

Link to publication in Scopus

Cite this

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title = "Imaging glucoamylase by scanning tunnelling microscopy",

abstract = "Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.",

author = "Gunning, {A. Patrick} and Morris, {Victor J.} and Gary Williamson and Belshaw, {Nigel J.} and Kramer, {Gerard F H} and Kanning, {Marja W.}",

year = "1994",

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doi = "10.1039/AN9941901939",

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volume = "119",

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T1 - Imaging glucoamylase by scanning tunnelling microscopy

AU - Gunning, A. Patrick

AU - Morris, Victor J.

AU - Williamson, Gary

AU - Belshaw, Nigel J.

AU - Kramer, Gerard F H

AU - Kanning, Marja W.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

AB - Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

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EP - 1942

JO - Analyst

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SN - 0003-2654

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