Imaging glucoamylase by scanning tunnelling microscopy

A. Patrick Gunning, Victor J. Morris, Gary Williamson, Nigel J. Belshaw, Gerard F H Kramer, Marja W. Kanning

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6 Citations (Scopus)

Abstract

Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

Original languageEnglish
Pages (from-to)1939-1942
Number of pages4
JournalAnalyst
Volume119
Issue number9
DOIs
Publication statusPublished - 1 Dec 1994
Externally publishedYes

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