Lactococcus lactis strain plasma intake suppresses the incidence of dengue fever-like symptoms in healthy malaysians: A randomized, double-blind, placebo-controlled trial

Chee Sieng Khor, Ryohei Tsuji, Hai Yen Lee, Siti Sarah Nor’e, Norhidayu Sahimin, Adzzie Shazleen Azman, Vunjia Tiong, Pouya Hasandarvish, Boon Teong Teoh, Yih Harng Soh, Jian Hai Chai, Takeshi Kokubo, Osamu Kanauchi, Naoki Yamamoto, Sazaly Abubakar

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Dengue fever (DF) is a mosquito-borne disease still with no effective treatment or vaccine available. A randomized, placebo-controlled, double-blinded, parallel-group trial was undertaken to evaluate the efficacy of oral intake of Lactococcus lactis strain plasma (LC-Plasma) on the presentation and severity of DF-like symptoms among healthy volunteers. Study participants (320) were assigned into two groups, and consumed either placebo or LC-Plasma tablets (approximately 100 billion cells/day) for 8 weeks. The clinical symptoms of DF were self-recorded through questionnaires, and exposure to DENV was determined by serum antibody and/or DENV antigen tests. No significant differences between groups were observed for exposure to DENV, or the symptomatic ratio. Results obtained showed that participants from the LC-Plasma group reported a significant reduction in the cumulative incidence days of DF-like symptoms, which include fever (p < 0.001), muscle pain (p < 0.005), joint pain (p < 0.001), and pain behind the eyes (p < 0.001), compared to that of the placebo group. Subgroup analysis revealed a significantly (p < 0.05) reduced severity score in the LC-Plasma group when study sites were separately analyzed. Overall, our findings suggest that LC-Plasma supplementation reduces the cumulative days with DF-like symptoms, and the severity of the symptoms. Daily oral intake of LC-Plasma, hence, is shown to mitigate the DF-like symptoms.

Original languageEnglish
Article number4507
Number of pages15
Issue number12
Publication statusPublished - Dec 2021
Externally publishedYes


  • Dengue fever
  • Dengue virus
  • Lactic acid bacteria
  • Lactococcus lactis
  • LC-Plasma

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