IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells

Ngan Le; Ian Luk; David Chisanga; Wei Shi; Lokman Pang; Glen Scholz; John Mariadason; Matthias Ernst; Jennifer Huynh

doi:10.1016/j.cyto.2022.155887

IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells

Ngan Le, Ian Luk, David Chisanga, Wei Shi, Lokman Pang, Glen Scholz, John Mariadason, Matthias Ernst, Jennifer Huynh

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

Interleukin-36 gamma (IL-36G) is a member of the IL-36 subfamily of cytokines and acts as a potent driver of inflammation. IL-36G has been extensively characterized in the pathogenesis of psoriasis and has been recently described to play roles in wound healing particularly in the gastrointestinal tract. However, the effects of IL-36G during cancer development including gastric cancer remain unexplored. Here, we show that IL-36G induced ERK1/2 activation in AGS, MKN1 and MKN45 human gastric cancer cell lines. Moreover, IL-36G induced colony formation, migration and invasion of these gastric cancer cell lines that was inhibited by the natural antagonist, IL-36 receptor antagonist (RA). Interrogation of TCGA stomach adenocarcinoma patient datasets revealed highly elevated IL-36G gene expression in human gastric cancer compared to normal tissue independent of tumor stage, and high IL-36G expression corresponded with poorer patient survival. Collectively, our results indicate for the first time that IL-36G supports a neoplastic phenotype in human gastric cancer cells.

Original language	English
Article number	155887
Number of pages	6
Journal	Cytokine
Volume	155
DOIs	https://doi.org/10.1016/j.cyto.2022.155887
Publication status	Published - Jul 2022
Externally published	Yes

Keywords

Cytokines
Gastric cancer
IL-36G
Interleukins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cyto.2022.155887

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title = "IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells",

abstract = "Interleukin-36 gamma (IL-36G) is a member of the IL-36 subfamily of cytokines and acts as a potent driver of inflammation. IL-36G has been extensively characterized in the pathogenesis of psoriasis and has been recently described to play roles in wound healing particularly in the gastrointestinal tract. However, the effects of IL-36G during cancer development including gastric cancer remain unexplored. Here, we show that IL-36G induced ERK1/2 activation in AGS, MKN1 and MKN45 human gastric cancer cell lines. Moreover, IL-36G induced colony formation, migration and invasion of these gastric cancer cell lines that was inhibited by the natural antagonist, IL-36 receptor antagonist (RA). Interrogation of TCGA stomach adenocarcinoma patient datasets revealed highly elevated IL-36G gene expression in human gastric cancer compared to normal tissue independent of tumor stage, and high IL-36G expression corresponded with poorer patient survival. Collectively, our results indicate for the first time that IL-36G supports a neoplastic phenotype in human gastric cancer cells.",

keywords = "Cytokines, Gastric cancer, IL-36G, Interleukins",

author = "Ngan Le and Ian Luk and David Chisanga and Wei Shi and Lokman Pang and Glen Scholz and John Mariadason and Matthias Ernst and Jennifer Huynh",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2022",

month = jul,

doi = "10.1016/j.cyto.2022.155887",

language = "English",

volume = "155",

journal = "Cytokine",

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TY - JOUR

T1 - IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells

AU - Le, Ngan

AU - Luk, Ian

AU - Chisanga, David

AU - Shi, Wei

AU - Pang, Lokman

AU - Scholz, Glen

AU - Mariadason, John

AU - Ernst, Matthias

AU - Huynh, Jennifer

PY - 2022/7

Y1 - 2022/7

N2 - Interleukin-36 gamma (IL-36G) is a member of the IL-36 subfamily of cytokines and acts as a potent driver of inflammation. IL-36G has been extensively characterized in the pathogenesis of psoriasis and has been recently described to play roles in wound healing particularly in the gastrointestinal tract. However, the effects of IL-36G during cancer development including gastric cancer remain unexplored. Here, we show that IL-36G induced ERK1/2 activation in AGS, MKN1 and MKN45 human gastric cancer cell lines. Moreover, IL-36G induced colony formation, migration and invasion of these gastric cancer cell lines that was inhibited by the natural antagonist, IL-36 receptor antagonist (RA). Interrogation of TCGA stomach adenocarcinoma patient datasets revealed highly elevated IL-36G gene expression in human gastric cancer compared to normal tissue independent of tumor stage, and high IL-36G expression corresponded with poorer patient survival. Collectively, our results indicate for the first time that IL-36G supports a neoplastic phenotype in human gastric cancer cells.

AB - Interleukin-36 gamma (IL-36G) is a member of the IL-36 subfamily of cytokines and acts as a potent driver of inflammation. IL-36G has been extensively characterized in the pathogenesis of psoriasis and has been recently described to play roles in wound healing particularly in the gastrointestinal tract. However, the effects of IL-36G during cancer development including gastric cancer remain unexplored. Here, we show that IL-36G induced ERK1/2 activation in AGS, MKN1 and MKN45 human gastric cancer cell lines. Moreover, IL-36G induced colony formation, migration and invasion of these gastric cancer cell lines that was inhibited by the natural antagonist, IL-36 receptor antagonist (RA). Interrogation of TCGA stomach adenocarcinoma patient datasets revealed highly elevated IL-36G gene expression in human gastric cancer compared to normal tissue independent of tumor stage, and high IL-36G expression corresponded with poorer patient survival. Collectively, our results indicate for the first time that IL-36G supports a neoplastic phenotype in human gastric cancer cells.

KW - Cytokines

KW - Gastric cancer

KW - IL-36G

KW - Interleukins

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U2 - 10.1016/j.cyto.2022.155887

DO - 10.1016/j.cyto.2022.155887

M3 - Article

C2 - 35512531

AN - SCOPUS:85129530189

SN - 1043-4666

VL - 155

JO - Cytokine

JF - Cytokine

M1 - 155887

ER -

IL-36G promotes cancer-cell intrinsic hallmarks in human gastric cancer cells

Abstract

Keywords

UN SDGs

Access to Document

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