Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 294 - 298 |
| Number of pages | 5 |
| Journal | Pediatric Research |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 |
| Externally published | Yes |
Cite this
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IL-1alpha causes lung inflammation and maturation by direct effects on preterm fetal lamb lungs. / Sosenko, Ilene; Kallapur, Suhas; Nitsos, Ilias; Moss, Timothy; Newnham, John; Ikegami, Machiko; Jobe, Alan.
In: Pediatric Research, Vol. 60, No. 3, 2006, p. 294 - 298.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - IL-1alpha causes lung inflammation and maturation by direct effects on preterm fetal lamb lungs
AU - Sosenko, Ilene
AU - Kallapur, Suhas
AU - Nitsos, Ilias
AU - Moss, Timothy
AU - Newnham, John
AU - Ikegami, Machiko
AU - Jobe, Alan
PY - 2006
Y1 - 2006
N2 - Intra-amniotic endotoxin induces IL-1, causes chorioamnionitis, lung inflammation, lung injury and lung maturation in preterm lambs. Intra-amniotic IL-1alpha also causes chorioamnionitis, lung inflammation and lung maturation. We asked if IL-1alpha effects on the preterm lung are mediated by direct signaling to the lung rather than by indirect effects from the chorioamnionitis. To study IL-1 effects independently of chorioamnionitis, the lungs and the amniotic fluid were surgically separated in fetal sheep by diverting fetal lung fluid via a tracheostomy tube to a sialastic bag. A mini-osmotic pump delivered an intratracheal infusion of recombinant sheep IL-1alpha (10 microg) or saline (control) over 24 h. Preterm lambs were delivered 1d or 7d after the start of the infusion at 124d gestational age (Term = 150d). IL-1alpha recruited inflammatory cells and increased pro-inflammatory cytokine mRNA expression in the fetal lungs. Compared with controls, IL-1alpha did not alter lung antioxidant enzyme activity or alveolar numbers. IL-1alpha had minimal effects on the mRNA or protein expression of proteins essential for vascular development. IL-1alpha induced large increases in alveolar surfactant saturated phosphatidylcholine and increased lung gas volumes. Lung inflammation and maturation result from direct exposure of the fetal lung to a single cytokine - IL-1alpha.
AB - Intra-amniotic endotoxin induces IL-1, causes chorioamnionitis, lung inflammation, lung injury and lung maturation in preterm lambs. Intra-amniotic IL-1alpha also causes chorioamnionitis, lung inflammation and lung maturation. We asked if IL-1alpha effects on the preterm lung are mediated by direct signaling to the lung rather than by indirect effects from the chorioamnionitis. To study IL-1 effects independently of chorioamnionitis, the lungs and the amniotic fluid were surgically separated in fetal sheep by diverting fetal lung fluid via a tracheostomy tube to a sialastic bag. A mini-osmotic pump delivered an intratracheal infusion of recombinant sheep IL-1alpha (10 microg) or saline (control) over 24 h. Preterm lambs were delivered 1d or 7d after the start of the infusion at 124d gestational age (Term = 150d). IL-1alpha recruited inflammatory cells and increased pro-inflammatory cytokine mRNA expression in the fetal lungs. Compared with controls, IL-1alpha did not alter lung antioxidant enzyme activity or alveolar numbers. IL-1alpha had minimal effects on the mRNA or protein expression of proteins essential for vascular development. IL-1alpha induced large increases in alveolar surfactant saturated phosphatidylcholine and increased lung gas volumes. Lung inflammation and maturation result from direct exposure of the fetal lung to a single cytokine - IL-1alpha.
UR - http://www.nature.com/pr/journal/v60/n3/full/pr2006240a.html
U2 - 10.1203/01.pdr.0000233115.51309.d3
DO - 10.1203/01.pdr.0000233115.51309.d3
M3 - Article
VL - 60
SP - 294
EP - 298
JO - Pediatric Research
JF - Pediatric Research
SN - 0031-3998
IS - 3
ER -