IL-17A mediates a selective gene expression profile in asthmatic human airway smooth muscle cells

Stephane Dragon, Stuart J Hirst, Tak H Lee, Abdelilah Soussi Gounni

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12 Citations (Scopus)

Abstract

Airway smooth muscle (ASM) cells are thought to contribute to the pathogenesis of allergic asthma by orchestrating and perpetuating airway inflammation and remodeling responses. In this study, we evaluated the IL-17RA signal transduction and gene expression profile in ASM cells from subjects with mild asthma and healthy individuals. Human primary ASM cells were treated with IL-17A and probed by the Affymetrix GeneChip array, and gene targets were validated by real-time quantitative RT-PCR. Genomic analysis underlined the proinflammatory nature of IL-17A, as multiple NF-kappaB regulatory factors and chemokines were induced in ASM cells. Transcriptional regulators consisting of primary response genes were overrepresented and displayed dynamic expression profiles. IL-17A poorly enhanced IL-1beta or IL-22 gene responses in ASM cells from both subjects with mild asthma and healthy donors. Interestingly, protein modifications to the NF-kappaB regulatory network were not observed after IL-17A stimulation, although oscillations in IkappaBepsilon expression were detected. ASM cells from subjects with mild asthma up-regulated more genes with greater overall variability in response to IL-17A than from healthy donors. Finally, in response to IL-17A, ASM cells displayed rapid activation of the extracellular signal-regulated kinase/ribosomal S6 kinase signaling pathway and increased nuclear levels of phosphorylated extracellular signal-regulated kinase. Taken together, our results suggest that IL-17A mediated modest gene expression response, which, in cooperation with the NF-kappaB signaling network, may regulate the gene expression profile in ASM cells.
Original languageEnglish
Pages (from-to)1053 - 1063
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume50
Issue number6
DOIs
Publication statusPublished - 2014

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