In vivo fate of carbon nanotubes with different physicochemical properties for gene delivery applications

A Cifuentes-Rius, N R B Boase, I Font, N Coronas, V Ramos-Perez, Kristofer J. Thurecht, S Borrós

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Gene therapy has arisen as a pioneering technique to treat diseases by direct employment of nucleic acids as medicine. The major historical problem is to develop efficient and safe systems for the delivery of therapeutic genes into the target cells. Carbon nanotubes (CNTs) have demonstrated considerable promise as delivery vectors due to their (i) high aspect ratio and (ii) capacity to translocate through plasma membranes, known as the nanoneedle effect. To leverage these advantages, close attention needs to be paid to the physicochemical characteristics of the CNTs used. CNTs with different diameters (thinner and thicker) were treated by chemical oxidation to produce shorter fragments. Rigid (thick) and flexible (thin) CNTs, and their shortened versions, were coated with polyallylamine (ppAA) by plasma-enhanced chemical vapor deposition. The ppAA coating leads to a positively charged CNT surface that is able to electrostatically bind the green fluorescent protein plasmid reporter. This study shows how rigidity and length can affect their (i) behavior in biological media, (ii) ability to transfect in vitro, and (iii) biodistribution in vivo. This study also generates a set of basic design rules for the development of more efficient CNT-based gene-delivery vectors.

Original languageEnglish
Pages (from-to)11461-11471
Number of pages11
JournalACS Applied Materials & Interfaces
Volume9
Issue number13
DOIs
Publication statusPublished - 5 Apr 2017

Keywords

  • biodistribution
  • carbon nanotube rigidity
  • carbon nanotubes
  • cytotoxicity
  • gene transfection

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