TY - JOUR
T1 - In vitro elimination of EL4 cancer cells from spermatogonia stem cells by miRNA-143- A nd 206-loaded folic acid-conjugated PLGA nanoparticles
AU - Shams, Azar
AU - Shabani, Ronak
AU - Asgari, Hamidreza
AU - Karimi, Mahdi
AU - Najafi, Mohammad
AU - Asghari-Jafarabadi, Mohammad
AU - Razavi, Seyed Mohsen
AU - Miri, Seyed Rouhollah
AU - Abbasi, Mehdi
AU - Mohammadi, Amirhossein
AU - Koruji, Morteza
N1 - Funding Information:
This research was funded by a grant from the Iran University of Medical Sciences (IUMS), (grant no. 96-01-30-29861). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
Publisher Copyright:
© 2022 Future Medicine Ltd.
PY - 2022/4
Y1 - 2022/4
N2 - Aim: MiRNA's-143 and-206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- A nd 206-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. Materials & methods: The therapy was conducted in six groups: Treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. Results: In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. Conclusion: MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable.
AB - Aim: MiRNA's-143 and-206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- A nd 206-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. Materials & methods: The therapy was conducted in six groups: Treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. Results: In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. Conclusion: MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable.
KW - apoptosis
KW - cancer
KW - miRNA
KW - nanoparticle
KW - spermatogonia stem cell
UR - https://www.scopus.com/pages/publications/85127609365
U2 - 10.2217/nnm-2021-0210
DO - 10.2217/nnm-2021-0210
M3 - Article
C2 - 35264013
AN - SCOPUS:85127609365
SN - 1743-5889
VL - 17
SP - 531
EP - 545
JO - Nanomedicine
JF - Nanomedicine
IS - 8
ER -
SDG 3 Good Health and Well-being