In situ quantification of β-carotene partitioning in oil-in-water emulsions by confocal Raman microscopy

W A Fahmi Wan Mohamad, Roman Buckow, Mary Ann Augustin, Don McNaughton

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9 Citations (Scopus)

Abstract

Confocal Raman microscopy (CRM) was able to quantify the β-carotene concentration in oil droplets and determine the partitioning characteristics of β-carotene within the emulsion system in situ. The results were validated by a conventional method involving solvent extraction of β-carotene separately from the total emulsion as well as the aqueous phase separated by centrifugation, and quantification by absorption spectrophotometry. CRM also enabled the localization of β-carotene in an emulsion. From the Raman image, the β-carotene partitioning between the aqueous and oil phases of palm olein-in-water emulsions stabilized by whey protein isolate (WPI) was observed. Increasing the concentration of β-carotene in an emulsion (from 0.1 to 0.3 g/kg emulsion) with a fixed gross composition (10% palm olein:2% WPI) decreased the concentration of β-carotene in the oil droplet. CRM is a powerful tool for in situ analyses of components in heterogeneous systems such as emulsions.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalFood Chemistry
Volume233
DOIs
Publication statusPublished - 15 Oct 2017

Keywords

  • Confocal
  • Emulsion
  • Partitioning
  • Raman
  • Whey protein
  • β-Carotene

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