IgE-mediated enhancement of CD4+T cell responses requires antigen presentation by CD8α- conventional dendritic cells

Zhoujie Ding, Joakim S. Dahlin, Hui Xu, Birgitta Heyman

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15 Citations (Scopus)


IgE, forming an immune complex with small proteins, can enhance the specific antibody and CD4+T cell responses in vivo. The effects require the presence of CD23 (Fcϵ-receptor II)+ B cells, which capture IgE-complexed antigens (Ag) in the circulation and transport them to splenic B cell follicles. In addition, also CD11c+ cells, which do not express CD23, are required for IgE-mediated enhancement of T cell responses. This suggests that some type of dendritic cell obtains IgE-Ag complexes from B cells and presents antigenic peptides to T cells. To elucidate the nature of this dendritic cell, mice were immunized with ovalbumin (OVA)-specific IgE and OVA, and different populations of CD11c+ cells, obtained from the spleens four hours after immunization, were tested for their ability to present OVA. CD8α- conventional dendritic cells (cDCs) were much more efficient in inducing specific CD4+T cell proliferation ex vivo than were CD8α+ cDCs or plasmacytoid dendritic cells. Thus, IgE-Ag complexes administered intravenously are rapidly transported to the spleen by recirculating B cells where they are delivered to CD8α- cDCs which induce proliferation of CD4+T cells.

Original languageEnglish
Article number28290
Number of pages11
JournalScientific Reports
Publication statusPublished - 16 Jun 2016
Externally publishedYes

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