Abstract
| Original language | English |
|---|---|
| Article number | 481 |
| Number of pages | 3 |
| Journal | BMC Research Notes |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
Cite this
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Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples. / Mahfuz, Istiak; Cheng, Wei; White, Stefan.
In: BMC Research Notes, Vol. 6, No. 1, 481, 2013.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples
AU - Mahfuz, Istiak
AU - Cheng, Wei
AU - White, Stefan
PY - 2013
Y1 - 2013
N2 - BACKGROUND: The use of buccal swabs in clinical and scientific studies is a very popular method of collecting DNA, due to its non-invasive nature of collection. However, contamination of the DNA sample may interfere with analysis. FINDINGS: Here we report the finding of Streptococcus parasanguinis bacterial DNA contamination in human buccal DNA samples, which led to preferential amplification of bacterial sequence with PCR primers designed against human sequence. CONCLUSION: Contamination of buccal-derived DNA with bacterial DNA can be significant, and may influence downstream genetic analysis. One needs to be aware of possible bacterial contamination when interpreting abnormal findings following PCR amplification of buccal swab DNA samples.
AB - BACKGROUND: The use of buccal swabs in clinical and scientific studies is a very popular method of collecting DNA, due to its non-invasive nature of collection. However, contamination of the DNA sample may interfere with analysis. FINDINGS: Here we report the finding of Streptococcus parasanguinis bacterial DNA contamination in human buccal DNA samples, which led to preferential amplification of bacterial sequence with PCR primers designed against human sequence. CONCLUSION: Contamination of buccal-derived DNA with bacterial DNA can be significant, and may influence downstream genetic analysis. One needs to be aware of possible bacterial contamination when interpreting abnormal findings following PCR amplification of buccal swab DNA samples.
UR - http://www.biomedcentral.com/content/pdf/1756-0500-6-481.pdf
U2 - 10.1186/1756-0500-6-481
DO - 10.1186/1756-0500-6-481
M3 - Article
VL - 6
JO - BMC Research Notes
JF - BMC Research Notes
SN - 1756-0500
IS - 1
M1 - 481
ER -