Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples

Istiak Mahfuz, Wei Cheng, Stefan White

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND: The use of buccal swabs in clinical and scientific studies is a very popular method of collecting DNA, due to its non-invasive nature of collection. However, contamination of the DNA sample may interfere with analysis. FINDINGS: Here we report the finding of Streptococcus parasanguinis bacterial DNA contamination in human buccal DNA samples, which led to preferential amplification of bacterial sequence with PCR primers designed against human sequence. CONCLUSION: Contamination of buccal-derived DNA with bacterial DNA can be significant, and may influence downstream genetic analysis. One needs to be aware of possible bacterial contamination when interpreting abnormal findings following PCR amplification of buccal swab DNA samples.
Original languageEnglish
Article number481
Number of pages3
JournalBMC Research Notes
Volume6
Issue number1
DOIs
Publication statusPublished - 2013

Cite this

Mahfuz, Istiak ; Cheng, Wei ; White, Stefan. / Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples. In: BMC Research Notes. 2013 ; Vol. 6, No. 1.
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Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples. / Mahfuz, Istiak; Cheng, Wei; White, Stefan.

In: BMC Research Notes, Vol. 6, No. 1, 481, 2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of Streptococcus parasanguinis DNA contamination in human buccal DNA samples

AU - Mahfuz, Istiak

AU - Cheng, Wei

AU - White, Stefan

PY - 2013

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N2 - BACKGROUND: The use of buccal swabs in clinical and scientific studies is a very popular method of collecting DNA, due to its non-invasive nature of collection. However, contamination of the DNA sample may interfere with analysis. FINDINGS: Here we report the finding of Streptococcus parasanguinis bacterial DNA contamination in human buccal DNA samples, which led to preferential amplification of bacterial sequence with PCR primers designed against human sequence. CONCLUSION: Contamination of buccal-derived DNA with bacterial DNA can be significant, and may influence downstream genetic analysis. One needs to be aware of possible bacterial contamination when interpreting abnormal findings following PCR amplification of buccal swab DNA samples.

AB - BACKGROUND: The use of buccal swabs in clinical and scientific studies is a very popular method of collecting DNA, due to its non-invasive nature of collection. However, contamination of the DNA sample may interfere with analysis. FINDINGS: Here we report the finding of Streptococcus parasanguinis bacterial DNA contamination in human buccal DNA samples, which led to preferential amplification of bacterial sequence with PCR primers designed against human sequence. CONCLUSION: Contamination of buccal-derived DNA with bacterial DNA can be significant, and may influence downstream genetic analysis. One needs to be aware of possible bacterial contamination when interpreting abnormal findings following PCR amplification of buccal swab DNA samples.

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DO - 10.1186/1756-0500-6-481

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JO - BMC Research Notes

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