Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots

Wee Kiat Ong; Chuen Seng Tan; Kai Li Chan; Grace Gandi Goesantoso; Xin Hui Derryn Chan; Edmund Chan; Jocelyn Yin; Chia Rou Yeo; Chin Meng Khoo; Jimmy Bok Yan So; Asim Shabbir; Sue Anne Toh; Weiping Han; Shigeki Sugii

doi:10.1016/j.stemcr.2014.01.002

Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots

Wee Kiat Ong, Chuen Seng Tan, Kai Li Chan, Grace Gandi Goesantoso, Xin Hui Derryn Chan, Edmund Chan, Jocelyn Yin, Chia Rou Yeo, Chin Meng Khoo, Jimmy Bok Yan So, Asim Shabbir, Sue Anne Toh, Weiping Han, Shigeki Sugii

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133 Citations (Scopus)

Abstract

Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

Original language	English
Pages (from-to)	171-179
Number of pages	9
Journal	Stem Cell Reports
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2014.01.002
Publication status	Published - 11 Feb 2014
Externally published	Yes

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10.1016/j.stemcr.2014.01.002

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Ong, W. K., Tan, C. S., Chan, K. L., Goesantoso, G. G., Chan, X. H. D., Chan, E., Yin, J., Yeo, C. R., Khoo, C. M., So, J. B. Y., Shabbir, A., Toh, S. A., Han, W., & Sugii, S. (2014). Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots. Stem Cell Reports, 2(2), 171-179. https://doi.org/10.1016/j.stemcr.2014.01.002

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title = "Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots",

abstract = "Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.",

author = "Ong, {Wee Kiat} and Tan, {Chuen Seng} and Chan, {Kai Li} and Goesantoso, {Grace Gandi} and Chan, {Xin Hui Derryn} and Edmund Chan and Jocelyn Yin and Yeo, {Chia Rou} and Khoo, {Chin Meng} and So, {Jimmy Bok Yan} and Asim Shabbir and Toh, {Sue Anne} and Weiping Han and Shigeki Sugii",

note = "Funding Information: We thank members of Laboratory of Metabolic Medicine, Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium-Nikon Imaging Centre, Biopolis Shared Facility Flow Cytometry Unit, and Toh{\textquoteright}s lab at National University of Singapore for helping our research activities. S.S. is a cofounder of LipoStation Pte., Ltd., which has had no financial or scientific influence on this study. This work is supported by an intramural funding from Biomedical Research Council of Agency for Science, Technology and Research (A ∗ STAR) to W.H. and S.S. and funding from the Singapore National Medical Research Council to S.-A.T. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.stemcr.2014.01.002",

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AU - Ong, Wee Kiat

AU - Tan, Chuen Seng

AU - Chan, Kai Li

AU - Goesantoso, Grace Gandi

AU - Chan, Xin Hui Derryn

AU - Chan, Edmund

AU - Yin, Jocelyn

AU - Yeo, Chia Rou

AU - Khoo, Chin Meng

AU - So, Jimmy Bok Yan

AU - Shabbir, Asim

AU - Toh, Sue Anne

AU - Han, Weiping

AU - Sugii, Shigeki

N1 - Funding Information: We thank members of Laboratory of Metabolic Medicine, Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium-Nikon Imaging Centre, Biopolis Shared Facility Flow Cytometry Unit, and Toh’s lab at National University of Singapore for helping our research activities. S.S. is a cofounder of LipoStation Pte., Ltd., which has had no financial or scientific influence on this study. This work is supported by an intramural funding from Biomedical Research Council of Agency for Science, Technology and Research (A ∗ STAR) to W.H. and S.S. and funding from the Singapore National Medical Research Council to S.-A.T. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 2014/2/11

Y1 - 2014/2/11

N2 - Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

AB - Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

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ER -

Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots

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