Identification of pathways mediating growth differentiation factor5-induced tenogenic differentiation in human bone marrow stromal cells

Sik-Loo Tan; Tunku Sara Ahmad; Wuey Min Ng; Azlina Amir Abbas; Azhar Mahmood Merican; Lakshmi Selvaratnam; Tunku Kamarul Zaman

doi:10.1371/journal.pone.0140869

Identification of pathways mediating growth differentiation factor5-induced tenogenic differentiation in human bone marrow stromal cells

Sik-Loo Tan, Tunku Sara Ahmad, Wuey Min Ng, Azlina Amir Abbas, Azhar Mahmood Merican, Lakshmi Selvaratnam, Tunku Kamarul Zaman

Malaysia Jeffrey Cheah Sch of Med & HS

Research output: Contribution to journal › Article › Research › peer-review

32 Citations (Scopus)

Abstract

To date, the molecular signalling mechanisms which regulate growth factors-induced MSCs tenogenic differentiation remain largely unknown. Therefore, a study to determine the global gene expression profile of tenogenic differentiation in human bone marrow stromal cells (hMSCs) using growth differentiation factor 5 (GDF5) was conducted. Microarray analyses were conducted on hMSCs cultures supplemented with 100 ng/ml of GDF5 and compared to undifferentiated hMSCs and adult tenocytes. Results of QuantiGene? Plex assay support the use and interpretation of the inferred gene expression profiles and pathways information. From the 27,216 genes assessed, 873 genes (3.21 of the overall human transcriptome) were significantly altered during the tenogenic differentiation process (corrected p

Original language	English
Pages (from-to)	1 - 22
Number of pages	22
Journal	PLoS ONE
Volume	10
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0140869
Publication status	Published - 2015

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Cite this

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abstract = "To date, the molecular signalling mechanisms which regulate growth factors-induced MSCs tenogenic differentiation remain largely unknown. Therefore, a study to determine the global gene expression profile of tenogenic differentiation in human bone marrow stromal cells (hMSCs) using growth differentiation factor 5 (GDF5) was conducted. Microarray analyses were conducted on hMSCs cultures supplemented with 100 ng/ml of GDF5 and compared to undifferentiated hMSCs and adult tenocytes. Results of QuantiGene? Plex assay support the use and interpretation of the inferred gene expression profiles and pathways information. From the 27,216 genes assessed, 873 genes (3.21 of the overall human transcriptome) were significantly altered during the tenogenic differentiation process (corrected p",

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AU - Tan, Sik-Loo

AU - Ahmad, Tunku Sara

AU - Ng, Wuey Min

AU - Abbas, Azlina Amir

AU - Merican, Azhar Mahmood

AU - Selvaratnam, Lakshmi

AU - Kamarul Zaman, Tunku

PY - 2015

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AB - To date, the molecular signalling mechanisms which regulate growth factors-induced MSCs tenogenic differentiation remain largely unknown. Therefore, a study to determine the global gene expression profile of tenogenic differentiation in human bone marrow stromal cells (hMSCs) using growth differentiation factor 5 (GDF5) was conducted. Microarray analyses were conducted on hMSCs cultures supplemented with 100 ng/ml of GDF5 and compared to undifferentiated hMSCs and adult tenocytes. Results of QuantiGene? Plex assay support the use and interpretation of the inferred gene expression profiles and pathways information. From the 27,216 genes assessed, 873 genes (3.21 of the overall human transcriptome) were significantly altered during the tenogenic differentiation process (corrected p

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