Identification of pathways mediating growth differentiation factor5-induced tenogenic differentiation in human bone marrow stromal cells

Sik-Loo Tan, Tunku Sara Ahmad, Wuey Min Ng, Azlina Amir Abbas, Azhar Mahmood Merican, Lakshmi Selvaratnam, Tunku Kamarul Zaman

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29 Citations (Scopus)


To date, the molecular signalling mechanisms which regulate growth factors-induced MSCs tenogenic differentiation remain largely unknown. Therefore, a study to determine the global gene expression profile of tenogenic differentiation in human bone marrow stromal cells (hMSCs) using growth differentiation factor 5 (GDF5) was conducted. Microarray analyses were conducted on hMSCs cultures supplemented with 100 ng/ml of GDF5 and compared to undifferentiated hMSCs and adult tenocytes. Results of QuantiGene? Plex assay support the use and interpretation of the inferred gene expression profiles and pathways information. From the 27,216 genes assessed, 873 genes (3.21 of the overall human transcriptome) were significantly altered during the tenogenic differentiation process (corrected p
Original languageEnglish
Pages (from-to)1 - 22
Number of pages22
JournalPLoS ONE
Issue number11
Publication statusPublished - 2015

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