To evaluate the utility of an enhanced biomarker discovery approach in order to identify potential biomarkers relevant to ovarian cancer detection. Experimental design: We combined immuno-depletion, liquid-phase IEF, 1D-DIGE, MALDI-TOF/MS and LC-MS/MS to identify differentially expressed proteins in the plasma of symptomatic ovarian cancer patients, stratified by stage, compared to samples obtained from normal subjects.
Shield-Artin, K. L., Bailey, M. J., Oliva, K., Lovic, A. K., Barker, G., Dellios, N. L., Reisman, S., Ayhan, M., & Rice, G. E. (2012). Identification of ovarian cancer-associated proteins in symptomatic women: A novel method for semi-quantitative plasma proteomics. Proteomics - Clinical Applications, 6(3-4), 170 - 181. https://doi.org/10.1002/prca.201100008