Identification of novel immunogens in Pasteurella multocida

Keith Al-Hasani, John Dallas Boyce, Victoria McCarl, Stephen Paul Bottomley, Ian W Wilkie, Ben Adler

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27 Citations (Scopus)

Abstract

P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only limited protection against homologous serotypes. Therefore there is a need for more effective vaccines to control diseases caused by P. multocida. As a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. Results: Bioinformatics analysis of the P. multocida genome predicted 129 proteins as secreted, located in the outer membrane, or lipoproteins. 105 of the genes encoding these proteins were cloned and recombinant protein expressed in Escherichia coli. Polyclonal serum from P. multocida-infected chickens reacted with a subset of these proteins. Conclusion: These data show the range of bacterial immunogens recognized by the chicken immune system, including 6 novel immunoreactive proteins.
Original languageEnglish
Pages (from-to)1 - 5
Number of pages5
JournalMicrobial Cell Factories
Volume6
Issue number3
Publication statusPublished - 2007

Cite this

Al-Hasani, K., Boyce, J. D., McCarl, V., Bottomley, S. P., Wilkie, I. W., & Adler, B. (2007). Identification of novel immunogens in Pasteurella multocida. Microbial Cell Factories, 6(3), 1 - 5.