TY - JOUR
T1 - Identification of novel immunogens in Pasteurella multocida
AU - Al-Hasani, Keith
AU - Boyce, John Dallas
AU - McCarl, Victoria
AU - Bottomley, Stephen Paul
AU - Wilkie, Ian W
AU - Adler, Ben
PY - 2007
Y1 - 2007
N2 - P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only limited protection against homologous serotypes. Therefore there is a need for more effective vaccines to control diseases caused by P. multocida. As a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. Results: Bioinformatics analysis of the P. multocida genome predicted 129 proteins as secreted, located in the outer membrane, or lipoproteins. 105 of the genes encoding these proteins were cloned and recombinant protein expressed in Escherichia coli. Polyclonal serum from P. multocida-infected chickens reacted with a subset of these proteins. Conclusion: These data show the range of bacterial immunogens recognized by the chicken immune system, including 6 novel immunoreactive proteins.
AB - P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only limited protection against homologous serotypes. Therefore there is a need for more effective vaccines to control diseases caused by P. multocida. As a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. Results: Bioinformatics analysis of the P. multocida genome predicted 129 proteins as secreted, located in the outer membrane, or lipoproteins. 105 of the genes encoding these proteins were cloned and recombinant protein expressed in Escherichia coli. Polyclonal serum from P. multocida-infected chickens reacted with a subset of these proteins. Conclusion: These data show the range of bacterial immunogens recognized by the chicken immune system, including 6 novel immunoreactive proteins.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17233917
M3 - Article
SN - 1475-2859
VL - 6
SP - 1
EP - 5
JO - Microbial Cell Factories
JF - Microbial Cell Factories
IS - 3
ER -
